Abstract: Taking the evanescent-wave optical fiber fluorescent biosensor as a platform and the phthalic acid esters（PAEs）contaminants as targets,the biosensing analytical technology based on the estrogen receptor interaction theory was optimized,and qualitative screening of estrogen binding activity of PAEs was achieved. The order of the estrogenic binding activity of seven typical PAEs were BBP > DBP > DIPP. The other 4substances had almost no estrogenic binding activity,which was consistent with the widely reported conclusions in Literatures,and confirming the accuracy of the established method. Under the optimal test conditions,the optical fiber sensing surface can be regenerated more than 300 times,providing a Low-cost,automated method for the screening of estrogenic contaminants.

Key words: evanescent-wave fiber fluorescent biosensor, estrogen receptor, phthalates, estrogenic binding activity