Establishment of the Real-time and Label-free Screening System for Tumor Cell Apoptosis

doi:10.13560/j.cnki.biotech.bull.1985.2019-0399

Abstract

Abstract: A real-time and label-free tumor cell apoptosis screening system via combining real-time xCELLigence-RTCA analysis system and flow cytometry technology. Cisplatin was used to induce the apoptosis of non-small lung cancer cells A549,and xCELLigence-RTCA system through 96 h continuous monitoring was applied to acquire the dynamic growth curve of A549 cells. The optimal testing time of apoptosis and 50% inhibitory concentration was deduced from the growth curve. Combined with precise quantitative flow cytometry technology,apoptotic rate of A549 cells treated with cisplatin was analyzed. These results confirmed the feasibility and reliability of the real-time and free technology for apoptosis screening of tumor cells.

Key words: apoptosis detection, real-time and label-free technology, flow cytometry, effective drug concentration

LI Yan-wei, SONG Xing-hui, WANG Jia-jia, LIU Li, HUANG Ying-ying, GUO Chun. Establishment of the Real-time and Label-free Screening System for Tumor Cell Apoptosis[J]. Biotechnology Bulletin, 2019, 35(10): 220-226.

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