[1] Saiki RK, Scharf S, Faloona F, et al. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia[J]. Science, 1985, 230(4732_:1350-1354. [2] Mullis K, Faloona F, Scharf S, et al. Specific enzymatic amplification of DNA in vitro:the polymerase chain reaction[J]. Cold Spring Harbor Symposia on Quantitative Biology, 1986, 51 Pt 1:263-273. [3] Kammann M, Laufs J, Schell J, et al.Rapid insertional mutagenesis of DNA by polymerase chain reaction(PCR)[J]. Nucleic Acids Res, 1989, 17(13):5404. [4] Lu Y, Xiao S, Yuan M, et al.Using overlap-extension PCR technique to fusing genes for constructing recombinant plasmids[J]. Journal of Basic Microbiology, 2018, 58(3):273-276. [5] Hussain H, Chong NF.Combined overlap extension PCR method for improved site directed mutagenesis[J]. BioMed Research International, 2016:8041532. [6] 雒丽娜, 王盛, 王玉炯. 利用重叠延伸PCR技术进行定点突变研究[J]. 安徽农业科学, 2012(10):5779-5781. [7] 徐书景, 张跃灵, 张妍, 等. 改进重叠延伸PCR技术构建定点双突变[J]. 中国生物工程杂志, 2010(10):49-54. [8] 谢振华, 史小军, 蔡国平. 在单个PCR管内快捷完成定点突变[J]. 中国生物化学与分子生物学报, 2006(8):681-684. [9] 赵松子, 沈向群. 用滚环扩增与大引物PCR法高效构建定点突变序列[J]. 江西农业学报, 2009(8):7-8. [10] 张碧乾, 邓会群, 宫彩霞, 等. 大引物PCR定点突变方法的改进[J]. 湖北大学学报:自然科学版, 2009(1):79-81. [11] 谢振华, 史小军. 快捷的定点突变效率近100%的大引物PCR方法(英文)[J]. 生物化学与生物物理进展, 2009, 36(11):1490-1494. [12] 莫秋华, 徐湘民, 钟雄霖, 等. 一种改进的大引物PCR定点诱变方法[J]. 中华医学遗传学杂志, 2002(1):72-75. [13] Gao A, Sun T, Ma G, et al.LEM4 confers tamoxifen resistance to breast cancer cells by activating cyclin D-CDK4/6-Rb and ERalpha pathway[J]. Nature Communications, 2018, 9(1):4180. [14] Li Z, Razavi P, Li Q, et al.Loss of the FAT1 Tumor suppressor promotes resistance to CDK4/6 inhibitors via the Hippo pathway[J]. Cancer Cell, 2018, 34(6):893-905. [15] Zhong S, Zhu J, Li Y, et al.Butylene fipronil induces apoptosis in PC12 murine nervous cells via activation of p16-CDK4/6-cyclin D1 and mitochondrial apoptotic pathway[J]. Journal of Biochemical and Molecular Toxicology, 2018:e22264. [16] Lee Y, Dominy JE, Choi YJ, et al.Cyclin D1-CDK4 controls glucose metabolism independently of cell cycle progression[J]. Nature, 2014, 510(7506):547-551. [17] Ruas M, Gregory F, Jones R, et al.CDK4 and CDK6 delay senescence by kinase-dependent and p16INK4a-independent mechanisms[J]. Molecular and Cellular Biology, 2007, 27(12):4273-4282. [18] Upender M, Raj L, Weir M.Megaprimer method for in vitro mutagenesis using parallel templates[J]. Biotechniques, 1995, 18(1):29-30, 32. [19] Maschmann A, Kounovsky-Shafer KL.Determination of restriction enzyme activity when cutting DNA labeled with the TOTO dye family[J]. Nucleosides Nucleotides Nucleic Acids, 2017, 36(6):406-417. [20] Chaudhary VK, Shrivastava N, Verma V, et al.Rapid restriction enzyme-free cloning of PCR products:a high-throughput method applicable for library construction[J]. PLoS One, 2014, 9(10):e111538. |