Inhibitory Effects of AI-2 Quorum Sensing Inhibitors from Marine Lactic Acid Bacteria on Listeria monocytogenes

doi:10.13560/j.cnki.biotech.bull.1985.2018-0934

Abstract

Abstract: The biofilm and virulence factors regulated by AI-2（Autoinducer 2）quorum sensing system are the main causes of high morbidity and mortality of Listeria monocytogenes. In this experiment,the luminescence value of the reporter Vibrio harveyi BB170 was used as the index to screen the AI-2 quorum sensing inhibitors（QSIs）of L. monocytogene from the metabolites of 16 marine lactic acid bacteria. The control effect of QSIs on L. m was evaluated by measuring its MIC value,growth curve,dynamic formation,and biofilm formation. The results showed that the ethyl acetate extracts from 6 strains of 16 marine lactic acid bacteria had a promising inhibitory effect on the AI-2 signaling molecule activity of L. monocytogene,accounting for 37.5% of the screened strains,and the inhibition rate reached over 75 %. Among them,the ethyl acetate extracts from strain Pediococcus pentosaceus zy-B-1 presented the highest inhibition rate（98.5%）and the MIC value to L. monocytogene was 250 μg/mL. As the concentration of QSI-B-1 increased,the inhibitory effect on the growth and dynamic formation of L. monocytogene was gradually strengthened,and the biofilm structure became more and more loose. This study provides a basis for screening the QSIs of L. monocytogene from the marine lactic acid bacteria.

Key words: marine lactic acid bacteria, Listeria monocytogenes, quorum sensing inhibitor, AI-2

HUANG Xiang-mei, WU Ya-qian, LIU Ying, LIANG Jia-ye, SU Wei-ming. Inhibitory Effects of AI-2 Quorum Sensing Inhibitors from Marine Lactic Acid Bacteria on Listeria monocytogenes[J]. Biotechnology Bulletin, 2019, 35(4): 36-42.

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