Establishment and Performance Evaluation of Nano-fluorescence Strip for Detecting CSFV Antibody

doi:10.13560/j.cnki.biotech.bull.1985.2019-0986

Abstract

Abstract: An immunochromatography method for the detection of the antibodies against classical swine fever virus（CSFV）was established based on lanthanide europium microsphere labeling technology. By optimizing the coating concentration,resolving concentration and reaction time in the reaction system,the optimal reaction conditions were determined and the detection method was established. Then the performance of the system was evaluated from sensitivity,specificity,precision and clinical evaluation. The optimized reaction system was as that the concentration of coating was at 0.1 mg/mL,the dilution parameter was 6 times,and the detection time was 15 min. From the performance evaluation of the strips,the sensitivity of the strip for the detection of CSFV antibody was that the diluted 128 times of national reference samples of swine fever positive serum were still detected,and no cross-reactions were observed with the positive sera against common CSFV,for example PPRSV,PRV,FMDV,PCV,PEDV,BVDV,and BDV. The coefficient of variation was < 10%. Compared with the commercial ELISA kit of CSFV antibody,the coincidence rate for positive was 90% and that for negative was 100%,and the total coincidence rate was 97.7%. Compared with FVNT,the coincidence rate for positive were 93% and that for negative was 100%,and the total coincidence rate was 98.5%. According to the above comprehensive evaluation,the fluorescence detection method of CSFV antibody established in this study conforms to all the performance parameters. It can be used for rapid,economical and convenient detection of CSFV antibody in pigs,and can be widely applied to pig farm management at the grass-roots units.

Key words: nano-fluorescence, classical swine fever virus, fluorescence immunochromatography

WU Hao-xing, LIU Bai-hong, LIU Xue-wei, ZHOU Jing-yun, MA Yong-ying, YANG Xin-yan, LI Bao-chun, CHEN Xi-zhao. Establishment and Performance Evaluation of Nano-fluorescence Strip for Detecting CSFV Antibody[J]. Biotechnology Bulletin, 2020, 36(5): 74-79.

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