Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (5): 191-200.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0991
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XU Chong-xin(), ZHANG Xiao, LIU Yuan(), ZHONG Jian-feng, XIE Ya-jing, LU Li-na, GAO Mei-jing, LIU Xian-jin()
Received:
2021-08-05
Online:
2022-05-26
Published:
2022-06-10
Contact:
LIU Yuan,LIU Xian-jin
E-mail:hhxyxcx@163.com;liuyuan@jaas.ac.cn;jaasliu@jaas.ac.cn
XU Chong-xin, ZHANG Xiao, LIU Yuan, ZHONG Jian-feng, XIE Ya-jing, LU Li-na, GAO Mei-jing, LIU Xian-jin. Screening and Identification of Humanized Genetically Engineered Antibody Targeting to Simulate the Anti-insect Function of Bt Cry1C Protein[J]. Biotechnology Bulletin, 2022, 38(5): 191-200.
筛选轮数 Biopanning round | 抗原包被浓度 Concentration of coated antigen/(μg·mL-1) | 投入噬菌体 Input of phage particles/(μg·mL-1) | 产出噬菌体 Output of phage particles/(μg·mL-1) | 投入产出比 Bound of phage particles/% |
---|---|---|---|---|
1 | 100 | 5.65×1010 | 3.32×106 | 5.88×10-3 |
2 | 50 | 4.91×1010 | 2.09×107 | 4.26×10-2 |
3 | 25 | 6.62×1010 | 1.96×109 | 2.96 |
4 | 10 | 7.06×1010 | 3.12×109 | 4.42 |
Table 1 Input and output of phage particles in each round of biopanning
筛选轮数 Biopanning round | 抗原包被浓度 Concentration of coated antigen/(μg·mL-1) | 投入噬菌体 Input of phage particles/(μg·mL-1) | 产出噬菌体 Output of phage particles/(μg·mL-1) | 投入产出比 Bound of phage particles/% |
---|---|---|---|---|
1 | 100 | 5.65×1010 | 3.32×106 | 5.88×10-3 |
2 | 50 | 4.91×1010 | 2.09×107 | 4.26×10-2 |
3 | 25 | 6.62×1010 | 1.96×109 | 2.96 |
4 | 10 | 7.06×1010 | 3.12×109 | 4.42 |
Fig. 7 Insecticidal effects of E8-Anti-Id scFv to C. medinalis and P. xylostella at 48 h indoor A:Insect used in the experiment is C. medinalis(CK- is PBS buffer. CK+ is Bt Cry1C protein solution dissolved in PBS buffer. E8 is phage-displayed E8-Anti-Id scFv solution dissolved in PBS buffer). B:Insect used in the experiment is P.xylostella(CK- is PBS buffer. CK+ is Bt Cry1C protein solution dissolved in PBS buffer. E8 is phage-displayed E8-Anti-Id scFv solution dissolved in PBS buffer)
供试样品 Samples for bioassay | 稻纵卷叶螟校正死亡率Corrected mortality for C. medinalis/% | 小菜蛾校正死亡率 Corrected mortality for P. xylostella/% | ||||
---|---|---|---|---|---|---|
24 h | 48 h | 72 h | 24 h | 48 h | 72 h | |
CK-(PBS solution) | 0.00±0.00a | 0.00±0.00a | 3.52±1.26a | 0.00±0.00a | 0.00±0.00a | 1.36±2.01a |
CK+(Bt Cry1C protein) | 37.21±4.26a | 65.56±3.77a | 72.15±1.36a | 35.16±1.12a | 69.25±4.13a | 75.36±1.29a |
E8(E8-Anti-Id scFv) | 29.83±3.13a | 44.32±2.09a | 52.82±4.79a | 26.38±1.01a | 48.99±1.23a | 58.69±2.32a |
Table 2 Insecticidal activities of E8-Anti-Id scFv to C. medinalis and P. xylostella indoor
供试样品 Samples for bioassay | 稻纵卷叶螟校正死亡率Corrected mortality for C. medinalis/% | 小菜蛾校正死亡率 Corrected mortality for P. xylostella/% | ||||
---|---|---|---|---|---|---|
24 h | 48 h | 72 h | 24 h | 48 h | 72 h | |
CK-(PBS solution) | 0.00±0.00a | 0.00±0.00a | 3.52±1.26a | 0.00±0.00a | 0.00±0.00a | 1.36±2.01a |
CK+(Bt Cry1C protein) | 37.21±4.26a | 65.56±3.77a | 72.15±1.36a | 35.16±1.12a | 69.25±4.13a | 75.36±1.29a |
E8(E8-Anti-Id scFv) | 29.83±3.13a | 44.32±2.09a | 52.82±4.79a | 26.38±1.01a | 48.99±1.23a | 58.69±2.32a |
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