Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (1): 31-39.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0339

Previous Articles     Next Articles

Spatio-temporal Expression of Cry1Ab/Cry2Aj Insecticidal Protein in Genetically Modified Maize Ruifeng 125 with Stacked Insect and Herbicide Resistance Traits

LI Dong-yang1,3(), XIAO Bing4, WANG Chen-yao1, YANG Xian-ming2, LIANG Jin-gang1(), WU Kong-ming2()   

  1. 1. Development Center of Science and Technology, Ministry of Agriculture and Rural Affairs, Beijing 100176
    2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193
    3. State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000
    4. Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081
  • Received:2022-03-22 Online:2023-01-26 Published:2023-02-02
  • Contact: LIANG Jin-gang,WU Kong-ming E-mail:hzaulidongyang@163.com;liangjingang@agri.gov.cn;wukongming@caas.cn

Abstract:

Maize is one of the most important food crops in China, but pests reduce the quality and yield of maize production. Genetically modified(GM)insect resistant maize expressing Bacillus thuringiensisBt)toxins are new tools for integrated pest management. The insecticidal effect of Bt-transgenic maize is closely related to the expression of exogenous insecticidal protein. Evaluating the expression of Bt insecticidal protein in different growth stages and tissues is of significance to the integrated pest management and biosafety management of agricultural GM organisms. In this paper, the expression of Cry1Ab/Cry2Aj insecticidal protein in different tissues of Ruifeng 125 planted in 9 places in two years was determined by ELISA. The Bt insecticidal protein expressions in the leaves at jointing stage(V6-V8), tassels at tasseling stage(VT), leaves and filaments at silking stage(R1), leaves and grains at maturity stage(R4)were comparatively analyzed. It was found that the expressions of Bt insecticidal protein in different tissues of maize varied largely. The results showed that the expression of Cry1Ab/Cry2Aj insecticidal protein in the leaves was higher than that in the kernels. The expressions of Cry1Ab/Cry2Aj insecticidal protein of Ruifeng 125 from 9 different places were different, but the overall difference was slight.

Key words: genetically modified insect-resistant Bt maize, Ruifeng 125, Cry1Ab/Cry2Aj insecticidal protein, spatio-temporal expression