Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (5): 32-43.doi: 10.13560/j.cnki.biotech.bull.1985.2022-1106

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Advances in Plant Extracellular Vesicles and Analysis Techniques

ZHANG Xue-ping1,2(), LU Yu-qing1,2, ZHANG Yue-qian1,2, LI Xiao-juan1,2()   

  1. 1. College of Biological Sciences and Technology, Beijing Forestry University, National Engineering Technology Center for Forest Breeding and Ecological Restoration, Key Laboratory of Forest and Flower Genetics and Breeding of the Ministry of Education, Beijing 100083
    2. Institute of Tree Development and Gene Editing, Beijing Forestry University, Beijing 100083
  • Received:2022-09-09 Online:2023-05-26 Published:2023-06-08
  • Contact: LI Xiao-juan E-mail:zhangxp97@bjfu.edu.cn;lixj@bjfu.edu.cn

Abstract:

Extracellular vesicles(EVs)are nanoscale vesicles with a phospholipid bilayer structure released by cells through exocytosis under physiological and pathological conditions. EVs, as carriers of information such as proteins, nucleic acids, lipids, and metabolites, can shuttle between cells to play function of material transfer and information exchange, thus they are important for intercellular communication. In recent years, the research of EVs in plant has also been deepening, and there have been great progress in their research and analysis technologies. In this review, we briefly introduce the composition of extracellular vesicles. Then we summarize the current progress in the biological functions of EVs in plants. Furthermore, we analyze the advantages and disadvantages of EV isolation and enrichment methods, as well as the application of EVs in situ imaging technology. Finally, we prospect the potential technologies for the research of plant EVs.

Key words: extracellular vesicles, function, isolation method, in situ detection