Advances in Plant Cell Wall Structure and Imaging Technology

doi:10.13560/j.cnki.biotech.bull.1985.2022-1347

Abstract

Abstract:

Plant cell wall is a complex cross-linking network that provide mechanical support for plant cells during growth, development and adaptation to a changing environment. It has the functions of regulating plant morphology, resisting stress, transporting water, etc. In addition, most of the biomass accumulated by plant photosynthesis is stored in the cell wall. Therefore, it is of great significance to study the composition and structure of the cell wall for better utilization of plant energy. The nano-microstructure of plant cell wall is one of the frontier hotspots in plant research. With the development of new imaging technologies, a large number of studies had been conducted on the composition and structure of the cell wall. In this work, we summarized the composition, structure, imaging technology and mechanical property of the plant cell wall, in order to provide new ideas for studying plant cell wall.

Key words: plant cell wall, composition and structure, imaging technology, mechanical property

ZHANG Man, ZHANG Ye-zhuo, HE Qi-zou-hong, E Yi-lan, LI Ye. Advances in Plant Cell Wall Structure and Imaging Technology[J]. Biotechnology Bulletin, 2023, 39(7): 113-122.

Figures/Tables 3

References 68

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技术 Technology	原理 Principle	优势 Advantages	不足 Disadvantages	参考文献 Reference
原子力显微镜 Atomic force microscope	探针与样品表面相互作用力变化来获取信息	具有原子级分辨率，样品不需要特殊处理，可在生理状态下观察	检测效率低，存在图像假象	[28⇓⇓⇓⇓⇓-34]
拉曼显微术 Raman microscopy	基于光与物质作用后发生的拉曼散射，能够获得样品分子和化学水平的结构信息	CRM非侵入性原位成像 CARS信号灵敏度增加 SRS成像快，信噪比高	CRM有荧光干扰，成像时间长 CARS具有非共振背景，信噪比低 SRS需要化学成分达到一定丰度	[35⇓⇓⇓⇓⇓⇓⇓-43]
点击化学 Click chemistry	通过简单的化学合成利用带有荧光染料的基团对多糖类似物进行标记	反应条件简单且反应迅速，可在活体状态下追踪多糖、脂质的行踪	反应过程需要铜催化，会对植物体具有毒害作用	[44-45]
傅里叶变换红外光谱技术 Fourier transform infrared spectroscopy	通过检测化合物的红外分子吸收光谱，从而对样品中的不同化合物进行鉴定	快速、无损、需样品量少	背景复杂，空间分辨率有限	[46⇓⇓⇓⇓⇓⇓⇓-54]

技术 Technology	原理 Principle	优势 Advantages	不足 Disadvantages	参考文献 Reference
原子力显微镜 Atomic force microscope	探针与样品表面相互作用力变化来获取信息	具有原子级分辨率，样品不需要特殊处理，可在生理状态下观察	检测效率低，存在图像假象	[28⇓⇓⇓⇓⇓-34]
拉曼显微术 Raman microscopy	基于光与物质作用后发生的拉曼散射，能够获得样品分子和化学水平的结构信息	CRM非侵入性原位成像 CARS信号灵敏度增加 SRS成像快，信噪比高	CRM有荧光干扰，成像时间长 CARS具有非共振背景，信噪比低 SRS需要化学成分达到一定丰度	[35⇓⇓⇓⇓⇓⇓⇓-43]
点击化学 Click chemistry	通过简单的化学合成利用带有荧光染料的基团对多糖类似物进行标记	反应条件简单且反应迅速，可在活体状态下追踪多糖、脂质的行踪	反应过程需要铜催化，会对植物体具有毒害作用	[44-45]
傅里叶变换红外光谱技术 Fourier transform infrared spectroscopy	通过检测化合物的红外分子吸收光谱，从而对样品中的不同化合物进行鉴定	快速、无损、需样品量少	背景复杂，空间分辨率有限	[46⇓⇓⇓⇓⇓⇓⇓-54]