Identification of Laccase （LAC） Gene Family in Potato （Solanum tuberosum L.） and Its Expression Analysis under Salt Stresses

doi:10.13560/j.cnki.biotech.bull.1985.2025-0337

Abstract

Abstract:

Objective Laccases (LACs) are a class of copper-containing polyphenol oxidases that play significant roles in plant growth, development, and stress responses. The aim of this study is to identify the members of the StLAC gene family in potato (Solanum tuberosum L.) and analyze their expression characteristics, providing a reference for the functional study of StLACs. Method Based on the potato genome data, the StLAC gene family in potato was identified using bioinformatics methods. The expression patterns of StLAC genes in different tissues and under abiotic stress were analyzed using transcriptome data, and the expression patterns of StLAC genes under salt stress were verified by RT-qPCR. Result A total of 77 StLAC genes were identified in potato. Among them, 56 StLAC proteins had an isoelectric point >7, and only StLAC69 was a hydrophobic protein, while the other 76 members were hydrophilic proteins. Subcellular localization predicted results indicated that 20 StLAC proteins were located in the chloroplast, 19 in the vacuole, and the remaining StLAC proteins were located in the extracellular space, cytoplasm, nucleus, and plasma membrane. Analysis of cis-acting elements showed that the promoter regions of the StLAC gene family contained elements related to light response, growth and development, hormones, and stress response. Transcriptome data analysis revealed that StLAC genes were specifically expressed in different tissues and were induced under salt, drought, and hormone stress. The expression patterns of 12 StLAC family members (StLAC4/12/17/26/36/40/43/45/55/56/66/68) in the roots and leaves under salt stress were verified by RT-qPCR. The results showed that the expressions of StLAC4 and StLAC56 in the roots were upregulated by 3.8 to 15 times after stress, and the expressions of StLAC12, StLAC40, and StLAC56 in the leaves were upregulated by 17 to 34 times. Conclusion The StLAC gene family in potato is large and diverse, and may play an important role in the response of potato to salt stress.

Key words: potato, laccase, salt stress, bioinformatics analysis, expression analysis

GONG Hui-ling, XING Yu-jie, MA Jun-xian, CAI Xia, FENG Zai-ping. Identification of Laccase （LAC） Gene Family in Potato （Solanum tuberosum L.） and Its Expression Analysis under Salt Stresses[J]. Biotechnology Bulletin, 2025, 41(9): 82-93.

Figures/Tables 9

Table 1 Primer information for RT-qPCR

基因 Gene	上游引物 Forward primer（5'-3'）	下游引物 Reverse primer（5'-3'）
StLAC4	GTCATTTGGATGTTCACTTGCCTTG	TCTGGTGGAGGAGGAGGTAGC
StLAC12	AGGTCCAGTTGTTTATGCTCAAGAAG	ACACGATAGCCTCTGACGGATG
StLAC17	GGTTGTTCCATTGCCACATTGATTC	CGGTTCCATTGTCTGTTTCTCTCC
StLAC26	AAGGTGATCGTCTTGAAATTGAAGTTG	GTGTTACATAGGCTGGTCCATCTG
StLAC36	ACCTTTGGCACCTCTTTGGAATG	GAGTAGGAGGCTTAGGAGACAACC
StLAC40	TGTCGTTTGTGCCACTTGATACTC	CCTGTATGCTTCCAACTCTGAATCC
StLAC43	ATCGTGTCTGCTACATTGCTCTG	CAAGAGGAGAAGCGGTGATATAAGAG
StLAC45	TGCTATCTTCTCTGCTTCACAACAC	TTCCAATCACCTGCTGCTTAACTC
StLAC55	GGGAAACACCGTTACGCCATC	CACCTTGTCCGCAACACCATAG
StLAC56	AGGCATATAATAGCGTAGTTGGAGTTG	GGGAGGATGGGTGGTGATGAAG
StLAC66	TGTCGTTTGTGCCACTTGATACTC	CCTGTATGCTTCCAACTCTGAATCC
StLAC68	TGCTATCTTCTCTGCTTCACAACAC	TTCCAATCACCTGCTGCTTAACTC

Fig. 1 Phylogenetic tree analysis of LAC genes from S. tuberosum (St) and A. thaliana (At)

Fig. 2 Phylogenetic tree (A), conserved motif structure(B), and gene structure(C) in StLAC gene family

Fig. 3 Chromosome location of the StLAC gene family in S. tuberosum

Fig. 4 Chromosomal collinear relationship between A. thaliana (At) and S. tuberosum (St) LAC genes

Fig. 5 Protein interactions network among members of the StLAC family

Fig. 6 Type and number of cis-acting elements in the promoter of StLAC gene family

Fig. 7 Expression analysis of LAC gene family of S.tuberosum in different tissues (A) and under different stress (B)

Fig. 8 Relative expressions of StLACs in S. tuberosum roots (A) and leaf (B) under salt stressData are mean (± SE) from three independent biological replicates. Different letters indicate significant differences at P < 0.05

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