Abstract:

The aim was to provide biomaterial, technique flow and protocol standard of transgenic zebrafish study via founding a platform of zebrafish transgenic technology. A couple of Tübingen zebrafish were multiplicated whose zygotes were microinjected with high active constitute plasmid pEGFP-N3. Injected eggs were collected and cultured and further observation. Fluorescent microscopic observation was followed by PCR on these embryos to screen and certify GFP expressed transgenic zebrafish. Results showed that the present study developed an intact cultivation system of zebrafish including its cultivation, reproduction and breeding. We also dug out the common emergency and proposed possible solutions during zebrafish maintenance. We designed the molecular processes with zebrafish embryo as material in our lab. Especially a platform of zebrafish transgenic technology was founded for the regeneration of transgenic zebrafish lines and study of transgene integration mechanism.

Key words: Danio rerio, Maintenance, Transgenic technology, Microinjection