Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (10): 127-130.

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Screening and Identification of Actinomyces Q14 Antagonistic Against Alternaria Alternata

Lian Lihui, Gao Lijun, Yang Na, Wang Tao, Zhang Xianzhong   

  1. （Taishan Medical College，Taian 271000）
  • Received:2013-03-25 Revised:2013-10-15 Online:2013-10-14 Published:2013-10-15

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Abstract:

It was to screen the new antagonistic actinomyces against Alternaria alternata. Totally 36 strains of actinomycetes were obtained from eggplant roots. By dual culture on PDA plates, it was found that 6 strains（16.67% of total isolates）of actinomycetes showed satisfactory antagonistic effects to Alternaria alternate. Among them, strain Q14 exhibited the strongest antibiosis capacity with an inhibitory zone of 14.5 mm, and also showed good antagonistic effects to Fusarium oxysporum f. sp. cucumerinum and Fusarium oxysporum f. sp. lycopersici.
Strain Q14 was identified as Streptomyces polychromogenes according to its 16S rDNA sequence（GenBank acc. No. KC675186）analysis, and the morphological, physiological and biochemical characteristics.

Key words: Alternaria alternate, Antagonistic actinomycetes, Streptomyces polychromogenes