Complete Gene Sequencing and Genetic Analysis of PCV2 Isolates from Guizhou Province

Abstract

Abstract: It was to get the molecular biology data of the PCV2 strains in Guizhou province, 3 isolations of PCV2(GZ-LZ1strain, GZ-KY1strain and GZ-PX1 strain)were cloned and sequenced. The sequences were then multiple-aligned with representative sequences published in GenBank, and phylogenetic analysis was performed, both using bioinfromatics software. Sequence analysis showed that complete gene of all the 3 PCV2 isolates were 1 767 bp, the accession numbers were, KC788503, KC788504and KC788505. In pairwise comparisons, they were closely relate to each other and to the strains of vaccine producted in China with high nucleotide sequence identity from 97.0% to 98.3% and 95.5%-98.2%, respectively. However, they were more divergent from those of other countries or orther regions of China with lower level of similarities from 77.5% to 99.9%. GZ-LZ1 and GZ-PX1 were closely relate to the the strainYM-SH of vaccine, the strain of GZ-KY1 Phylogenetic analysis revealed that all of the 3 isolates were in the branch of PCV2d, strains of was closely relate to the SC-10 strain, ZJ-38 strain and HLJ-10.

Key words: PCV2, Complete gene sequencing, Genetic analysis of isolates

Li Tao, Wang Kaigong, Cheng Zhentao, Zhou Bijun, Wen Ming , Cui Yalan. Complete Gene Sequencing and Genetic Analysis of PCV2 Isolates from Guizhou Province[J]. Biotechnology Bulletin, 2014, 0(3): 165-170.

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