Expression and Antiviral Activity of Chicken Interferon Alpha in Bombyx mori

Abstract

Abstract: Chicken interferon alpha plays a significant role in infection prevention and treatment of chicken virus diseases. The purpose of this study is to acquire efficient expression and production of biologically active chicken interferon alpha by the baculovirus expression vector system. The full length of chicken interferon alpha(ChIFN-α)gene was optimized and synthesized. The optimized ChIFNα was cloned into the baculovirus transfer vector pVL1393. Thus, the recombinant plasmid pVL-ChIFNα was constructed. The pVL-ChIFNα was co-transfected with the BmBacmid DNA. The hemolymph of silkworm larvae was collected after infected with the recombinant virus. Western blot analysis showed that chicken interferon-alpha protein with the molecular weight of 19 kD was expressed. The activity of the expressed chicken interferon-alpha was detected by inhibiting cytopathic effect in the Vero-VSV*GFP test system. The antiviral activity was about 3. 2×10 ⁵ U/mL. The chicken interferon alpha protein was successfully expressed in the baculovirus expression vector system, which would be valuable in developing efficient and cost effective chicken interferon biological product.

Key words: Chicken interferon alpha, Silkworm, Baculovirus expression vector system, Antiviral activity

Li Tiantian, Yang Ling, Yi Yongzhu, Shen Guifang, Zhang Zhifang, Li Yinü. Expression and Antiviral Activity of Chicken Interferon Alpha in Bombyx mori[J]. Biotechnology Bulletin, 2014, 0(3): 171-176.

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[1]	LI Dan, DU Meng-tan, XIU Ming-xia, LIU Xing-jian, ZHANG Zhi-fang, LI Yi-nv. Expression of Sheep Interferon Alpha in Silkworm and Determination of Its Activity Against Peste Des Petits Ruminants Virus [J]. Biotechnology Bulletin, 2022, 38(1): 187-193.
[2]	ZUO Zhen-yu, YU Fang, HUANG Yan-hong, WANG Jia-yi, LI Ling-ling. The Optimization of Ultrasound-assisted Mesothermal Degreasing Process of Silkworm Pupa [J]. Biotechnology Bulletin, 2016, 32(2): 158-164.
[3]	LIU Xing-jian, LI Hao-yang, HU Xiao-yuan, ZHANG Zhi-fang, YI Yong-zhu, LI Yi-nv. Expression and Antiviral Activity Detection of Porcine Interferon -gamma in Silkworm [J]. Biotechnology Bulletin, 2016, 32(1): 144-148.
[4]	Li Haoyang, Hu Xiaoyuan, Yi Yongzhu, Yang Xin, Zhang Zhifang, Li Yinü. Expression of Canine Interferon Alpha in Silkworm-baculovirus Expression System and the Antiviral Activity Assay [J]. Biotechnology Bulletin, 2015, 31(6): 216-220.
[5]	Wan Jing, Xiang Xingwei, Jiang Lingli, Zhou Xiangyang,. Advances on the Baculovirus Expression Vector System for Protein Complex Production [J]. Biotechnology Bulletin, 2014, 0(2): 7-14.