Optimization of the Synthesis of Alkyl Glucoside Catalyzed by Aspergillus aculeatus β-glucosidase-displaying Pichia pastoris Whole-cells

Abstract

Abstract: Aspergillus aculeatus β-glucosidase displayed Pichia pastoris cell surface was used as whole-cell biocatalyst, glucose as substrate, catalyzed synthesis alkyl glucosides of C6-C10 with reverse hydrolysis in water-alcohol two-phase system. The effects of various reaction factors, including water content, whole-cell biocatalyst dose, glucose concentration, pH and temperature were optimized. The catalytic effect of whole-cell biocatalyst were compared with commercial enzyme almond β-glucosidase and Novozym188 . The results showed that Novozym188 was not suitable for synthesis of APG, almond β-glucosidase need shorter time than whole-cell biocatalyst, but the final conversion of whole-cell biocatalyst was higher. In 5 mL total reaction volume, the optimal reaction conditions of HG：0.1 g glucose, 0.05 g whole-cells biocatalyst, 10% pH3.0 buffer；the optimal reaction conditions of OG：0.2 g glucose, 0.05 g whole-cells biocatalyst, 15% pH3.0 buffer；the optimal reaction conditions of DG：0.2 g glucose, 0.2 g whole-cells biocatalyst, 20% pH3.0 buffer, temperature 55℃, 200 r/min. After 72 h the maximum HG and DG yield could be 11.69% and 3.58%. The highest yield of OG was 6.34% after 96 h.

Key words: Cell surface displaying, β-glucosidase, Whole-cell biocatalyst, Alkyl glucosides

Hu Xiayan, Liu Duanyu, Zheng Suiping. Optimization of the Synthesis of Alkyl Glucoside Catalyzed by Aspergillus aculeatus β-glucosidase-displaying Pichia pastoris Whole-cells[J]. Biotechnology Bulletin, 2014, 0(6): 205-210.

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