Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (7): 86-92.

• Research Report • Previous Articles     Next Articles

Recombinase-mediated Marker Gene Excision in Transient Gene Expression System of Mesophyll Protoplasts

Chi Junjie1, 2, Dai Shaojun1, Wei Jianhua2, Wang Hongzhi2   

  1. 1. Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Harbin 150040;
    2. Beijing Agro-Biotechnology Research Center, Beijing 100097
  • Received:2014-01-27 Online:2014-07-15 Published:2014-07-16

Abstract: In the present study, we used transient gene expression system of Arabidopsis mesophyll protoplasts to test and compare site-specific recombination constructs, which the expression of recombinase was induced by the promoter for heat-shock proteins Hsp18.2, developed a simple and convenient system for rapid assessment of site-specific recombination constructs.