Cloning and Analysis of Cytosolic Glutamine Synthetase cDNA from Eichharnia crassipes

Abstract

Abstract: In this study, the template cDNA which was reversely transcribed from the total RNA of Eichharnia crassipes, was subjected to polymerase chain reaction（PCR）with the degenerate primers which was designed according to sequences of the Cytosolic Glutamine Synthetase（GS1）from other plants. The full-length cDNA sequence was achieved with Rapid Amplification of cDNA End method（RACE）from the amplification product. It includes 1434 bp with the open reading frame of 1071 bp which encoded 356 coding amino acids with a predicted size of 39.3 kD and a calculated pI of 5.52. The result of sequence homology analysis showed that the deduced protein had relatively high amino acid identity with GS1s from other plants. The prediction of sub-cellular localization showed that EcGS1 is a cytosolic glutamine synthetase.

Key words: Eichharnia crassipes, Glutamine synthetase, RACE, Cloning

Jiang Lihua, Fu Minghui, Li Yuanmei, Yan Guohua, Zheng Lijun, Cheng Xiaoli, Peng Jinping. Cloning and Analysis of Cytosolic Glutamine Synthetase cDNA from Eichharnia crassipes[J]. Biotechnology Bulletin, 2014, 0(7): 93-99.

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