Biotechnology Bulletin ›› 2014, Vol. 0 ›› Issue (8): 159-163.

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Breeding and Fermentation Characterization of Mutants of Bacillus licheniformis for 2，3-Butanediol Production

Guo Wenyi, Sun Qinghui, Song Lina, Gao Songsong, Yang Hongjiang   

  1. Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457
  • Revised:2014-01-23 Online:2014-08-15 Published:2014-08-01
  • Contact: 通讯作者: 杨洪江，男，博士，教授，研究方向：工业微生物育种；E-mail: hongjiangyang@tust.edu.cn

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Abstract:

Currently, most isolated 2,3-butanediol producing strains are pathogens and they cause certain risks to public health and environments. In this work, Bacillus sp. 127-7 was isolated for 2,3-butanediol（BD）production from raw milk samples. It was identified as Bacillus licheniformis by analyzing its 16S rRNA gene sequence. By UV-mutagenesis, mutants that could endure high glucose concentrations and produce relatively high amounts of acetoin were isolated for further analysis. In shake-flask fermentations, 2,3-BD production was increased by 41.1% in mutant strain BL41 compared with the parent strain 127-7. Additionally, BL41 yielded much less amounts of lactate acid in the cultures without acidity control and 2,3-BD reached 81.4 g/L in the fed-batch fermentation. Moreover, the minimal residual glucose concentration was incre ased to 30 g/L in the culture, the fermentation time was significantly reduced to 46 h with 83.4 g/L 2,3-BD. The highest productivity is up to 1.9 g/L·h. The results showed that B. licheniformis BL41 may be used as a candidate strain for industrial production of 2,3-butanediol.

Key words: Bacillus licheniformis, 2, 3-butanediol, UV-mutagenesis, Glucose tolerance