Cloning and Bioinformatics Analysis of the Phosphoenolpyruvate Carboxylase(Chpepc2)Gene from Marine Chlorella sp.

Abstract

Abstract: To further understand the characteristics of algae PEPCase, marine Chlorella sp. pepc2 gene was cloned and analyzed the structural and functional characteristics of marine Chlorella sp. PEPCase2 by bioinformatics method. The experimental results showed that, the cDNA sequence of marine Chlorella sp. pepc2 gene, compared with the cDNA sequence of Chlamydomonas reinhardtii pepc2 gene, the similarity was 90%, and its amino acid sequence has the same characteristics with C. reinhardtii PEPCase2, so this gene encodes the bacterial-type PEPCase of marine Chlorella sp.；The secondary structures of marine Chlorella sp. PEPCase2 mainly include α-helix, β-turn, random coli and extended strand, and its tertiary structure is a parallel β-barrel structure in the centre with α-helix structures outside；marine Chlorella sp. PEPCase2 has many important physiological functions, mainly related to a variety of important material synthesis.

Key words: Marine Chlorella sp., Phosphoenolpyruvate carboxylase, Bioinformatics , Structure , Function

Huang Xiwen,Shi Dingji,Jia Xiaohui,Tian Qilin,Jia Rui,He Peimin. Cloning and Bioinformatics Analysis of the Phosphoenolpyruvate Carboxylase(Chpepc2)Gene from Marine Chlorella sp.[J]. Biotechnology Bulletin, 2014, 0(11): 164-173.

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