MTMR2 Interacts with 14-3-3 and Regulates H2O2  Induced Cell Senescence

Abstract

Abstract: H₂O₂-induced cell senescence plays an important role in tumorigenesis. To further study the underlying mechanism, we identified five relevant members through a genome wide shRNA screening and took MTMR2（Myotubularin-related protein 2） as an example to figure out its function in H₂O₂-induced cell senescence. By plasmid construction and expression in combination with purification and mass spectrometry, we found a significant interacting protein of MTMR2：14-3-3 proteins family, and their interaction was then confirmed using biochemical methods.

Key words: H₂O₂, Cell senescence, MTMR2 , shRNA pool screening , shRNA

Li Chaoqun,Chen Yali,Xiao Dongguang,Pei Huadong. MTMR2 Interacts with 14-3-3 and Regulates H₂O₂ Induced Cell Senescence[J]. Biotechnology Bulletin, 2014, 0(11): 201-205.

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MTMR2 Interacts with 14-3-3 and Regulates H₂O₂ Induced Cell Senescence

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