Latest Research Progress on RNA Interference Technology

doi:10.13560/j.cnki.biotech.bull.1985.2017-0455

Abstract

Abstract: RNA interference（RNAi）is a phenomenon in which the target gene is silenced by double-stranded RNA（dsRNA）inducing homologous mRNA degradation in the eukaryotic cells. At present,RNAi technology,as an effective tool for gene regulation,is widely used in pest control,cure of tumor and cancer duo to its high specificity,high efficiency,transmission and other characteristics. Especially in the medical application,compared with the traditional vaccine prevention and drug therapy,RNAi technology has unparalleled advantages of target medication,reducing side effects and inhibiting the expression of virulence genes,thus it plays an increasing significant role in the new treatment methods of cancer and other diseases. The mechanism,introducing methods of RNAi and its latest application in plant improvement,biological control,insect resistance,disease prevention and treatment are synthetically analyzed and evaluated in this paper,for providing the reference for researchers in related fields.

Key words: RNAi, dsRNA, shRNA, transgenic method, action mechanism

WANG Wei-wei, LIU Ni, LU Qin, LING Xiao-fei, CHEN Hang. Latest Research Progress on RNA Interference Technology[J]. Biotechnology Bulletin, 2017, 33(11): 35-40.

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