Studies on Genetic Features of Sex Reversal in Cynoglossus semilaevis

doi:10.13560/j.cnki.biotech.bull.1985.2015.04.030

Biotechnology Bulletin ›› 2015, Vol. 31 ›› Issue (3): 207-212.doi: 10.13560/j.cnki.biotech.bull.1985.2015.04.030

Previous Articles Next Articles

Studies on Genetic Features of Sex Reversal in Cynoglossus semilaevis

Song Chao^1，2 Jiang Li² Wang Jingwei^2，3 Li Xiaofang⁴ Li Geng⁵ Zhang Xiaohui⁶ Wang Shu² Liu Zhe¹ Li Hengde²

(1. College of Animal Science and Technology，Gansu Agricultural University，Lanzhou 730070；2 BiotechnologyCenter，Chinese Academy of Fishery Sciences，Beijing 100141；3.College of Animal Science，Fujian Agricultural University，Fuzhou 350002；4. Zhongjie Tilapia Aquaculture Limited Company，Huanghua，Hebei 061108；5. Yingkou Proliferation Experimental Station，Chinese Academy of Fishery Sciences，Yingkou 115000；6. Research Center of Resources and Environment，Chinese Academy of Fishery Sciences，Beijing 100141)

Received:2014-07-18 Online:2015-03-16 Published:2015-03-16

Abstract

Abstract: The remarkable individual size differences of half-smooth tongue(Cynoglossus semilavi.)between female and male fishes exist. However, the lower ratio of female in cultured populations arise from sex reversal of the females leads to lower production efficiency. In sex-determination of some fishes and amphibians, sex reversal is interesting biology question and its molecular genetic mechanisms are rarely explored. In this study, 10 half-sib families are set up by utilizing two types of male parents：genetically males and pseudo males which are genetically females. The results showed that the females were all reversed into physiologically male fishes in two families with pseudo male parent. In the other 8 families with normal male parent, the ratio of sex reversal in individual populations presents continuous distribution, which fits for features of QTLs(Quantitative Trait Loci)typically；the heredity of sex reversal is lower, only 0.058. All of these results showed that, the pseudo males as parents, which demonstrate full paternal-effects, the female progenies were all reversed into pseudo males；interfamily selection for improving the genetic advances of sex ratio or genetic evaluation of sex reversal by using genetic markers are advantageous over intrafamily selection for lower heredity of sex-reversal；the continuous distribution of ratio of sex reversal implies the sex-determination for half-smooth tongue is depended on the interactions among multiple QTLs.

Key words: half-smooth tongue, sex-dertermination, sex-reversal

Song Chao, Jiang Li, Wang Jingwei, Li Xiaofang, Li Geng, Zhang Xiaohui, Wang Shu, Liu Zhe, Li Hengde. Studies on Genetic Features of Sex Reversal in Cynoglossus semilaevis[J]. Biotechnology Bulletin, 2015, 31(3): 207-212.

References

[1] 姜言伟, 万瑞景. 渤海半滑舌鳎早期形态及发育特征的研究[J]. 海洋水产研究, 1988(9)：193-201.
[2] 孟田湘, 任胜民. 渤海半滑舌鳎的年龄与生长[J]. 海洋水产研究, 1988, 9：173-183.
[3] 季相山, 陈松林, 马洪雨, 等. 半滑舌鳎养殖群体中自然性逆转伪雄鱼的发现[J]. 水产学报, 2010, 34(2)：322-327.
[4] Bull JJ. Evolution of sex determining mechanisms[M]. Benjamin/Cummings Publishing Company, Inc. , Menlo Park, CA. 1983.
[5] Stelkens RB, Wedekind C. Environmental sex reversal, Trojan sex genes, and sex ratio adjustment：conditions and population consequ-ences[J]. Molecular Ecology, 2010, 19(4)：627-646.
[6] Matsumoto Y, Crews D. Molecular mechanisms of temperature-dependent sex determination in the context of ecological developmental biology[J]. Molecular and Cellular Endocrinology, 2012, 354(1)：103-110.
[7] 周丽青, 杨爱国, 柳学周, 等. 半滑舌鳎染色体核型分析[J]. 水产学报, 2005, 29(3)：417-419.
[8] Chen S, Tian Y, Yang J, et al. Artificial gynogenesis and sex determ-ination in half-smooth tongue sole(Cynoglossus semilaevis)[J]. Marine Biotechnology, 2009, 11(2)：243-251.
[9] 李虎, 陈四清, 刘海金, 姜宏波, 王美玉. 半滑舌鳎养殖群体的性比与雌雄形态差异比较[J]. 水产学报, 2012, 36(9)：1331-1336.
[10] 梁卓, 陈松林, 张静, 等. 半滑舌鰨养殖群体雌雄比例与自然性逆转率[J]. 中国水产科学, 2013, 20(1)：44-49.
[11] 邓思平, 陈松林, 田永胜, 等. 半滑舌鳎的性腺分化和温度对性别决定的影响[J]. 中国水产科学, 2007, 14(5)：714-719.
[12] 张晓彦, 刘海金. 17β-雌二醇对半滑舌鳎性分化和生长的影响[J]. 东北农业大学学报, 2009, 40(6)：67-72.
[13] 程翠, 曲宪成. 黄鳝性逆转研究进展[J]. 湖南农业科学, 2010, (1)：121-124.
[14] 邢晨光, 金珊, 袁思平. 石斑鱼性逆转研究现状[J]. 水产科学, 2006, 25(4)：214-216.
[15] 刘绪生, 梁冰, 张树义. 黑鲷DMRT1基因cDNA的克隆、组织表达谱及在性别逆转前后性腺中的表达[J]. 动物学研究, 2004, 25(2)：158-161.
[16] Otake H, Shinomiya A, Matsuda M, et al. Wild-derived XY sex-reversal mutants in the medaka, Oryzias latipes[J]. Genetics, 2006, 173(4)：2083-2090.
[17] 马学坤, 柳学周, 温海深, 等. 半滑舌鳎性腺分化的组织学观察[J]. 海洋水产研究, 2006, 27(2)：55-61.
[18] 陈松林, 李仰真, 张静, 等. 半滑舌鳎快速生长及高雌性家系的筛选[J]. 水产学报, 2013, 37(4)：481-488.
[19] Chen SL, Ji XS, Shao CW, et al. Induction of mitogynogenetic diploids and identification of WW super-female using sex-specific SSR markers in half-smooth tongue sole(Cynoglossus semilaevis)[J]. Marine Biotechnology, 2012, 14(1)：120-128.
[20] Liao X, Ma HY, Xu GB, et al. Construction of a genetic linkage map and mapping of a female-specific DNA marker in half-smooth tongue sole(Cynoglossus semilaevis)[J]. Marine Biotechnology, 2009, 11(6)：699-709.
[21] 张志和, 沈富军, 孙姗, 等. 应用微卫星分型方法进行大熊猫父亲鉴定[J]. 遗传, 2003, 25(5)：504-510.
[22] Shao CW, Li QY, Chen SL, et al. Epigenetic modification and inheritance in sexual reversal of fish[J]. Genome Research, 2014, 24(4)：604-615.
[23] Chen SL, Zhang GJ, Shao CW, et al, Whole-genome sequence of a flatfish provides insights into ZW sex chromosome evolution and adaptation to a benthic lifestyle[J]. Nature Genetics, 2014, 46(3)：253-260.

Studies on Genetic Features of Sex Reversal in Cynoglossus semilaevis

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 0

Recommended Articles

Metrics

Comments