Application of 3 DNA Fingerprinting Techniques in Strain Identification

doi:10.13560/j.cnki.biotech.bull.1985.2015.06.012

Abstract

Abstract: Four strains of Geobacillus were analyzed with 3 DNA fingerprinting techniques of ERIC-PCR, BOX-PCR and RAPD for searching the proper strain identification measure for the genus especially Geobacillus stearothermophilus. Each of 4 strains of G. stearothermophilus had different fingerprint. The bands by simple and quick ERIC-PCR were quite clear, and the different strains could be recognized clearly. The bands by BOX-PCR were less, and the strains of the same bacteria could not be recognized clearly. More bands could be obtained by RAPD with higher identification；however the workload of identification and cost increased significantly. Three techniques may effectively identify the strains of Geobacillus, and ERIC-PCR is the most effective and fast method to identify strains of G. stearothermophilus.

Key words: strain identification, ERIC-PCR, BOX-PCR, RAPD, Geobacillus stearothermophilus

Liu Yi, Yao Su, Li Hui, Liu Yang, Liu Yong, Liu Bo, Cheng Chi. Application of 3 DNA Fingerprinting Techniques in Strain Identification[J]. Biotechnology Bulletin, 2015, 31(6): 81-86.

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