Screening and Identification of a Sponge-associated Fungus HMP-F66 Inducing Oxidative Burst in Tobacco Cell Suspensions

doi:10.13560/j.cnki.biotech.bull.1985.2015.07.026

Abstract

Abstract: The plant innate immune system provides an alternative way for crop protection, and the plant immunity triggers have been an important source of biorational pesticide leads. In order to find new plant resistance inducers, fifty sponge-associated fungal isolates were screened for their ability to induce oxidative burst in the tobacco suspensions using the H2DCF-DA fluorescence assay. Strain HMP-F66 showed significant activity in inducing H2O2 production in tobacco suspensions. HMP-F66 was assumed to be Aspergillus oryzae by its morphological, physiological, cultural characters, and rDNA ITS sequence analysis.

Key words: Aspergillus oryzae, Aspergillus flavus, Hymeniacidon perleve, oxidative burst, induced resistance

Pang Zhiwei, Lu Xu, Hu Jiangchun, Cheng Xiaoqi, Wang Nan, Song Yanling. Screening and Identification of a Sponge-associated Fungus HMP-F66 Inducing Oxidative Burst in Tobacco Cell Suspensions[J]. Biotechnology Bulletin, 2015, 31(7): 174-179.

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