Isolation，Identification and Phosphate-solubilizing Capacity of Phosphate-solubilizing Bacteria from the Rhizosphere of Camellia

doi:10.13560/j.cnki.biotech.bull.1985.2015.07.025

Abstract

Abstract: Phosphate-solubilizing bacteria were isolated from the rhizosphere of camellia, and 17 strains were screened using traditional method of isolation and culture of microorganisms. By utilizing the method of transparent zone, phosphate-solubilizing bacteria were preliminarily screened from the rhizosphere soil. Subsequently, the bacteria were re-screened by using method of molybdenum-antimony anti-spectrophotometric to measure content of soluble phosphorus in fermentation liquor, and strain 6-Y-09 with the highest capacity of solubilizing phosphate was gained. According to morphology of the colony, physiological and biochemical properties, 16S rDNA sequence and phylogenetic analysis, strain 6-Y-09 was identified as Burkholderia capacia. The strain has great potential in further development of microbiological fertilizer because of its stronger phosphate-solubilizing capability, which will provide an outstanding strain resource for improving phosphorus supply of camellia and promoting its growth.

Key words: camellia rhizosphere, phosphate-solubilizing bacteria, isolation and identification, Burkholderia capacia

Liu Xiaoyu, Fu Dengqiang, Chen Liangqiu, Yang Weibo, Li Dongxia, Fu Haiquan. Isolation，Identification and Phosphate-solubilizing Capacity of Phosphate-solubilizing Bacteria from the Rhizosphere of Camellia[J]. Biotechnology Bulletin, 2015, 31(7): 169-173.

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