Screening of a Strain Producing Acid-stable α-Amylase and Optimization of Its Fermentation Condition

doi:10.13560/j.cnki.biotech.bull.1985.2015.07.028

Abstract

Abstract: A strain SH3 producing acid-stable α-amylase was isolated from the soil nearing a starch factory. It was preliminarily identified as yeast. The pH range of crude enzyme reaction was 3. 8-8. 0 with the optimum of 5. 0, and the activity of the enzyme was still available in the temperature of 80℃ with the optimum of 50℃. The optimization of single-factor fermentation revealed that the optimal condition for the enzyme production was 37℃, pH 5. 0, soluble starch as carbon source, peptone as nitrogen source and 200 mL liquid volume. Orthogonal test showed that the optimal fermentation condition was as soluble starch of 15 g/L, peptone of 30 g/L, 37℃ and pH4. 5, under which the enzyme activity was 96.8 U/mg.

Key words: acid-stable α-amylase, strain screening, fermentation condition, process optimization, yeast

Qu Jianhang, Yin Yi, Jiao Guobao, Ding Changhe, Zhang Qian, Qu Lingbo, Liu Zhongmin. Screening of a Strain Producing Acid-stable α-Amylase and Optimization of Its Fermentation Condition[J]. Biotechnology Bulletin, 2015, 31(7): 188-192.

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