Detection of cry1Ab/Ac Gene and Protein Remained in Gut of Broiler Feeding Transgenic Rice

doi:10.13560/j.cnki.biotech.bull.1985.2015.09.011

Abstract

Abstract: To analyze the effects of genetically modified rice on feeding broilers, cry1Ab/Ac gene and protein remained in the jejunum, ileum, cecum and rectum of broiler feeding transgenic rice were detected, and the degradation patterns were testified in vitro. The results showed that there were no residues of cry1Ab/Ac gene in 4 intestinal parts of broilers fed transgenic rice for 21 and 42 d, and cry1Ab/Ac gene gradually degraded after 4 h in the gut in vitro test. No residues of Cry1Ab/Ac protein were detected in contents of the above 4 parts of broilers using ELISA and Western blot. In conclusion, cry1Ab/Ac gene and protein of the transgenic rice were easily digested and degraded in broiler intestines.

Key words: transgenic rice, broiler, gene and protein residues, digestion in vitro

Xu Deng, Geng Lili, Zhang Minhong, Lu Fan, Zhang Jie. Detection of cry1Ab/Ac Gene and Protein Remained in Gut of Broiler Feeding Transgenic Rice[J]. Biotechnology Bulletin, 2015, 31(9): 84-90.

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