Integration Analysis of Exogenous Gene and Line-Specific Detection in the Insect-Resistant cry1C-Transgenic Rice Jishengjing3

doi:10.13560/j.cnki.biotech.bull.1985.2018-0788

Abstract

Abstract: The flanking sequences of exogenous gene conferring insect-resistant cry1C-transgenic rice line Jishengjing3 and its inserted sites were analyzed by genome walking PCR,and a line-specific PCR detection method was established. T-DNA was found to be in nucleotides 2 790 685 to 2 790 589（GenBank accession number：NC_029257.1）in the chromosome 2 of Oryza Sativa（Japonica）genome after aligning the flanking sequences in left and right borders of Jishengjing3’s exogenous gene with the rice’s genome and T-DNA sequences. Based on the T-DNA insertion site,specific primers in the 2-sides region of the inserted site and the left border of T-DNA region were designed,and a event-specific PCR detection method for Jishengjing3 was established,which may provide accurate and rapid detection approach for identifying Jishengjing3.

Key words: insect-resistance cry1C-transgenic rice, flanking sequences, insertion site, event-specific detection

JIN Yong-mei, MA Rui, YU Zhi-jing, LIN Xiu-feng. Integration Analysis of Exogenous Gene and Line-Specific Detection in the Insect-Resistant cry1C-Transgenic Rice Jishengjing3[J]. Biotechnology Bulletin, 2019, 35(3): 6-12.

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