Cloning and Tissue Expression of Full-length cDNA in Gene Encoding Δ6-desaturase Fatty Acyl of Portunus trituberculatus

doi:10.13560/j.cnki.biotech.bull.1985.2015.09.019

Abstract

Abstract: The goal of this work is to investigate the self-synthesis capability of highly unsaturated fatty acid(HUFA)and understand the synthesis pathway of HUFA in Portunus trituberculatus. Full-length cDNA of gene for Δ6-desaturase fatty acyl in P. trituberculatus was cloned by rapid amplification of cDNA ends(RACE), and the expressions in 8 tissues such as hepatopancreas, intestine and gill etc. were analyzed by real-time quantitative PCR(qRT-PCR). Analysis of the full-length cDNA of gene for Δ6-desaturase fatty acyl revealed that it was 2 875 bp, including 465 bp of 5' UTR and 1 078 bp of 3' UTR, and encoded 443 amino acids. The presumed protein sequence had a typical desaturase structure：3 histidine-rich motifs, a b5 domain of an N terminal cytochrome and a heme-binding HPGG domain. qRT-PCR showed that gene for Δ6-desaturase fatty acyl expressed in all 8 tissues while the highest in hepatopancreas, less in intestine and muscle, and the least in the heart. The results indicate that P. trituberculatus possesses Δ6-desaturase fatty acyl.

Key words: Portunus trituberculatus, fatty acid desaturase, tissue expression analysis

Shi Qiuyan, Yang Zhigang, Wang Wei, Yao Qinqin, Wang Yao, Cheng Yongxu. Cloning and Tissue Expression of Full-length cDNA in Gene Encoding Δ6-desaturase Fatty Acyl of Portunus trituberculatus[J]. Biotechnology Bulletin, 2015, 31(9): 138-145.

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