Cloning and Tissue Expression Analysis of NITR in Nile Tilapia（Oreochromis niloticus）

doi:10.13560/j.cnki.biotech.bull.1985.2017.05.021

Abstract

Abstract: The encoding sequence of NITR（Novel immune-type receptor）gene（GenBank accession number：KX989509;designated as On-NITR）was cloned from the spleen of Nile tilapia（Oreochromis niloticus）. The complete cDNA sequence of On-NITR gene was 1 119 bp with an ORF of 1 026 bp encoding 341 amino acids. The deduced amino acid sequence of On-NITR had an estimated molecular weight of 37.38 kD and a theoretical pI of 8.28. Through NCBI BLAST,we found the amino acid sequence identities between On-NITR and previously reported fish NITRs were approximately 27% - 46%. Amino acid alignment indicated that On-NITR consisted of one typical signal peptide,two extracellular immunoglobulin（Ig）domains（V and V/C2）,one transmembrane domain,and one highly conserved the cytoplasmic region with an immunoreceptor tyrosine-based inhibitor motif（ITIM）and an ITIM-like motif（itim）. Moreover,the analysis by real time quantitative PCR revealed that the expression of On-NITR was detected in all examined tissues of a healthy Nile tilapia with the higher expression level in intestine,skin and liver,while lower expression in thymus,gill,spleen,heart,and brain as well as the lowest level in head and kidney.

Key words: Nile tilapia（Oreochromis niloticus）, NITR protein, gene cloning, tissue expression analysis

WANG Zhi-wen HUANG Yu ZHANG Hai-yan TANG Ju-fen JIAN Ji-chang LU Yi-shan. Cloning and Tissue Expression Analysis of NITR in Nile Tilapia（Oreochromis niloticus）[J]. Biotechnology Bulletin, 2017, 33(5): 145-152.

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