Gene Cloning and Cold-induced Expression Analysis of the Gene Encoding Gibberellin 20 Oxidase from Jatropha curcas

doi:10.13560/j.cnki.biotech.bull.1985.2015.10.023

Abstract

Abstract: Gibberellin（GA）20 oxidase, a rate-limiting enzyme in GA biosynthesis, determines the amount of active GA forms, GA₁ and GA₄. Based on our previous transcriptome data of chilling-hardened Jatropha curcas, a new gene encoding gibberellin 20 oxidase（named JcGA20ox）was cloned by RT-PCR from the root of J. curcas seedlings（GenBank accession number：KJ670150.1）. The full-length cDNA of JcGA20ox was 1 307 bp, containing an entire open reading frame（ORF）of 1 131 bp. This ORF encoded a polypeptide of 376 amino acids with the molecular weight of 43 kD and the pI value of 6.7. Bioinformatics analysis showed that the JcGA20ox protein contained a Fe²⁺ and 2-oxoglutarate dioxygenase domain（Fe2OG_OXY）, belonging to the 2-ODD family. Semi-quantitative RT-PCR analysis revealed that JcGA20ox expressed differently in different tissues, with abundant and remarkably cold-induced expression in stem, but scarcely in leaves.

Key words: Jatropha curcas, gibberellin 20 oxidase, 2-oxoglutarate dioxygenase domain, cold induction

Wang Haibo, Zou Zhurong, Gong Ming. Gene Cloning and Cold-induced Expression Analysis of the Gene Encoding Gibberellin 20 Oxidase from Jatropha curcas[J]. Biotechnology Bulletin, 2015, 31(10): 140-148.

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