Cloning and Prokaryotic Expression of Dd-Gpx from Ditylenchus destructor

doi:10.13560/j.cnki.biotech.bull.1985.2015.10.022

Abstract

Abstract: To further understand the structure and function of glutathione peroxidase, a glutathione peroxidase gene was cloned from plant-parasitic nematode（Ditylenchus destructor）using RACE method, and the gene（DdGpx）was transferred into vector of pET-41b to have the fusion expression. The full-length cDNA of DdGpx was 950 bp containing an open reading frame of 681 bp and encoding 226 amino acids. Bioinformatics analysis indicated that the N terminal of the protein had obvious signal peptide, belonging to the secretory protein. The prokaryotic expression vector pET-41b-DdGpx was constructed and then transformed into BL21（DE3）, there was a apparent band in 60 kD. Mass spectrum analysis indicated that there were 7 peptides matched with D. destructor GPX protein, implying that the recombinant protein of Dd-GPX was successfully expressed

Key words: Ditylenchus destructor, glutathione peroxidase, gene cloning, prokaryotic expression

Liu Yang, He Xufeng, Liu Jianxi, Ding Zhong. Cloning and Prokaryotic Expression of Dd-Gpx from Ditylenchus destructor[J]. Biotechnology Bulletin, 2015, 31(10): 131-139.

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