The Residue Detection of cry1Ab/Ac Gene and Protein in the Gut of Rattus norvegicus Feeding Transgenic Rice

doi:10.13560/j.cnki.biotech.bull.1985.2015.12.019

Abstract

Abstract:

The exogenous cry1Ab/Ac gene and protein residues in the jejunum, ileum, cecum of Rattus norvegicus feeding transgenic rice and parent rice were detected. The testing results showed that there were no residues of cry1Ab/Ac gene in 3 intestinal parts of R. norvegicus fed transgenic rice for 50 and 110 d, and overall length or fragment of exogenous cry1Ab/Ac gene gradually degraded after 4 h, and completely at 12 h in vitro. No residues of Cry1Ab/Ac protein were detected in contents of the above 3 parts of R. norvegicus using ELISA and Western blot method. In conclusion, the exogenous gene and protein of the transgenic rice were not remained in the intestines of R. norvegicus.

Key words: transgenic rice, Rattus norvegicus, gene and protein residues, digestion in vitro

Xu Deng, Geng Lili, Li Ning, Liu Xiaohui, Lu Fan, Zhang Jie. The Residue Detection of cry1Ab/Ac Gene and Protein in the Gut of Rattus norvegicus Feeding Transgenic Rice[J]. Biotechnology Bulletin, 2015, 31(12): 131-137.

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