Abstract: In order to establish the high frequencey regeneration system of Allium tuberosum, using the radicle as the explant, the paper examined callus and bud induction from its various parts. The results showed that the radicle tip was the best explant for inducing callus with the induction rate of 81.4%, and the bud induction rate reached 73.3%. Comparing the induction of callus and differentiation of bud in several media formulas, the regeneration rate was the highest in medium of MS+1 mg/L BA+0.5 mg/L NAA+0.5 mg/L KT, with induction rate over 80% and the differentiation rate of adventitious bud reaching 78.8%, respectively, while the propagation coefficient was 24.7. The percentage of adventitious buds elongating to be independent plant was 85.5% on elongation medium of MS+1 mg/L GA+0.5 mg/L KT at 30 d. The rooting rate and root system for the independent shoots were the best in the medium of 1/2 MS+0.3 mg/L IAA, and in which a single plant from the differentiation of radicle was generated at approximate 130 d.

Key words: Allium tuberosum, radicle, regeneration, plant regulators, shoot elongation