Cloning and Expression Analysis of Cystatin（HbCYS2）from Para Rubber Tree（Hevea brasiliensis）

doi:10.13560/j.cnki.biotech.bull.1985.2017.03.013

Abstract

Abstract: In plant,the cystatin（CYS）is widely involved in growth and development,and responses to biotic and abiotic stresses. In this study,the HbCYS2 is isolated from rubber tree,and has open reading frame（687 bp）encoding a protein of 288 aa. The HbCYS2 is a secreted protein with signal peptide,and has two CYS domains. The bioinformatics analysis indicates that the HbCYS2 is belonged to CYS II（GeneBank：KX161925）. The expression analysis indicated that the HbCYS2 has high level expression in latex,and similar expression pattern responding to tapping and ET treatment. In addition,the expression of HbCYS2 is apparently regulated by Corynespora inoculation in leaf. It is speculated that the HbCYS2 plays an important role in latex regeneration,tapping treatment and disease resistance in rubber tree.

Key words: Hevea brasiliensis, cystatin, latex regeneration, stress response

LONG Xiang-yu, LIANG Qi-fu, QI Ji-yan, Fang Yong-jun, TANG Chao-rong. Cloning and Expression Analysis of Cystatin（HbCYS2）from Para Rubber Tree（Hevea brasiliensis）[J]. Biotechnology Bulletin, 2017, 33(3): 86-92.

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