Biotechnology Bulletin ›› 2016, Vol. 32 ›› Issue (5): 151-157.doi: 10.13560/j.cnki.biotech.bull.1985.2016.05.020

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The Identification of a Fungal Endophyte of Suaeda salsa and Preliminarily Research on Active Products of Promoting Growth

YU Fei,GU Yue,ZHANG Qi,BU Ning   

  1. College of Chemistry and Life Sciences,Shenyang Normal University,Shenyang 110034
  • Received:2015-09-09 Online:2016-05-25 Published:2016-05-27

Abstract: The purposes of this work are to identify the endophyte fungus JP4-1 of Suaeda salsa in Red Beach of Panjin,Liaoning Province,and to research the growth promotion activity of its metabolites. Endophytic fungus JP4-1 was identified using its morphological features and 18S rDNA,and several technical means such as filtering centrifuge,ultrasonic crushing,extraction,and rotary evaporateion were used to extract its active components,and hydroponic experiments were taken to test the growth promotion activity of its fermentation broth to rice seedlings. The JP4-1 was identified as Glomerella sp. with hyphae branched,septate,and producing spores. The result of hydroponic experiment revealed that the micromolecular products extracted from fermented liquid with n-butanol promoted the plant height 10.84%,dry weight of leaves 14.67%,and chlorophyll content 44.22% of the rice seedlings,i.e.,the growth promotion effects were remarkable. In conclusion,the extracellular fermentation products by the endophyte fungus JP4-1 of S. salsa significantly promoted the growth of rice seedlings,and the micromolecular active products extracted by n-butanol had the most significant promotion effects.

Key words: fungal endophyte, identification, growth promotion activity