Study on the Highly Secreted Expression of the Recombinant Insulin in Pichia pastoris

doi:10.13560/j.cnki.biotech.bull.1985.2016.06.033

Biotechnology Bulletin ›› 2016, Vol. 32 ›› Issue (6): 226-230.doi: 10.13560/j.cnki.biotech.bull.1985.2016.06.033

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Study on the Highly Secreted Expression of the Recombinant Insulin in Pichia pastoris

LIANG Guo-yi¹, GAN Yi-di¹, LIU Xiao-hang¹, CAI Xiang-sheng²

1. Beijing SL Pharmaceutical Co.，Ltd.，Beijing 100043
2. Institute of Biotherapy of Southern Medical University，Guangzhou 510515

Received:2015-09-30 Online:2016-06-27 Published:2016-06-28

Abstract

Abstract: In order to improve the efficiency of insulin secretion expression in yeast，firstly the pro-insulin gene was synthesized，and a recombinant secreted expression vector(pPICZα-A-Pro-INS)was constructed by inserting a leader peptide to N-end of the gene’s sequence. Linearized expression vector was transformed into the competent cells of Pichia pastoris GSll5. A strain with highly secreted expression of the insulin was screened，by which the secreted recombinant protein expression level was 300 mg/L，accounting for about 40% of the total secreted protein. This result revealed that the leader peptide is crucial to the gene of highly expressing insulin in P. pastoris.

Key words: insulin, Pichia pastoris, highly expression, leader peptide

LIANG Guo-yi, GAN Yi-di, LIU Xiao-hang, CAI Xiang-sheng. Study on the Highly Secreted Expression of the Recombinant Insulin in Pichia pastoris[J]. Biotechnology Bulletin, 2016, 32(6): 226-230.

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Study on the Highly Secreted Expression of the Recombinant Insulin in Pichia pastoris

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