Identification of Aeromonas hydrophila and Histopathological Observation of Artificial Infected Zebrafish

doi:10.13560/j.cnki.biotech.bull.1985.2016.09.032

Abstract

Abstract: The aims of this paper are to isolate and identify pathogenic strain from diseased zebrafish,and to observe the histopathological changes of artificial infected zebrafish. The isolated strain ZF4 was identified by comprehensive analysis of its morphology,physiological and biochemical characteristics,conserved gene and phylogenetic tree. The results showed that the ZF4 was gram negative bacillus,positive in indole test,fermented glucose,sucrose sugars,etc. The gene AHA_0438,ASP,gyrB and 16S rRNA of ZF4 were all positive. Based on gyrB and 16S rRNA gene sequence,clustering analysis result showed that ZF4 was Aeromonas hydrophila. The infected zebrafish presented obvious clinical symptoms,and histopathological changes were observed,such as the liver tissue degeneration,intestinal lining shedding,hyperemia of the spleen,pancreas fibrous tissue hyperplasia.,etc. In conclusion,the ZF4 isolated from the diseased zebrafish was identified as pathogenicity A. hydrophila. Meanwhile,due to its susceptibility,zebrafish could be used as the animal model to study the A. hydrophila.

Key words: zebrafish, Aeromonas hydrophila, identification, artificial infection, histopathology

LIN Jin-xing¹, YANG Chi^{1, 2}, FENG Li-ping¹, HU Jian-hua¹. Identification of Aeromonas hydrophila and Histopathological Observation of Artificial Infected Zebrafish[J]. Biotechnology Bulletin, 2016, 32(9): 239-245.

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