Differential Expression Analysis of Gene Expression Profiles During Fruit Development and Ripening of Lycium ruthenicum

doi:10.13560/j.cnki.biotech.bull.1985.2016.11.017

Abstract

Abstract: The purpose of this study was to sequence the transcriptome at 3 different developmental stages of Lycium ruthenicum Murr. fruit,as well as analyze and compare the variations of their gene expression profiles. The RNAs of the fruits were extracted,and sequenced by Illumina high-throughput,then the their functions were annotated and classified using public database such as GO,KEGG,etc.,further their genes were compared and analyzed by digital gene expression profiles. Enrichment analysis results by KEGG pathway showed that in the “leucine biosynthesis pathway”,there were 7 genes differentially expressed in color changing vs early light green skin stage,while 35 differentially expressed genes in full ripening stage vs color changing stage,and all were up-regulated. The gene expressions of 3 rate-limiting enzymes in the non-reversible reactions of glycolysis all were up-regulated in the full ripening stage. Moreover,the functions and metabolic pathways of all genes involved in leucine biosynthesis and glycolysis of L. ruthenicum fruit were analyzed,aiming at providing the theoretical basis for exploring the germplasm of L. ruthenicum.

Key words: Lycium ruthenicum, fruit ripening, gene expression profile, differential expression

PENG Yong, CHEN Shang-wu, MA Hui-qin. Differential Expression Analysis of Gene Expression Profiles During Fruit Development and Ripening of Lycium ruthenicum[J]. Biotechnology Bulletin, 2016, 32(11): 144-151.

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