Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (10): 140-147.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0163
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YANG Hong-liang(), YUAN Zhen, QIAN Xu-jia-zhi, XU Da-wei()
Received:
2022-02-11
Online:
2022-10-26
Published:
2022-11-11
Contact:
XU Da-wei
E-mail:2629680722@qq.com;xudawei@sjtu.edu.cn
YANG Hong-liang, YUAN Zhen, QIAN Xu-jia-zhi, XU Da-wei. Expression Profile Analysis of Thionin-like Gene Family in Barley[J]. Biotechnology Bulletin, 2022, 38(10): 140-147.
基因名称Gene | 上游引物Forward primer(5'-3') | 下游引物Reverse primer(5'-3') |
---|---|---|
Thil1 | TCAGATTTTCACAAACCCGA | GACCGATTTGACTCAGACCA |
Thil2 | TCTCAGCCGTTGCCATCCTC | CCGCTCGCTCTCTACCTCAC |
Thil3 | TAAAGCCAAGAAGCCAACCA | CATTACAGAAACGGGCACAT |
Thil4 | CAAGCAACTCTCAAGAACAC | TTACAGTTACAGGCACACGA |
Thil5 | ATCTTAGAGCCATTCATTTC | TGACATATACTGGTCTTGGA |
Thil6 | ATAAATAGGAGCCTCGTGTT | CATTACAGAAACGGGCACAT |
Thil7 | CACCCAACCCTGACACACTC | CAGCAACTCTGAGGACGAAC |
Table 1 Sequences of qRT-PCR primers
基因名称Gene | 上游引物Forward primer(5'-3') | 下游引物Reverse primer(5'-3') |
---|---|---|
Thil1 | TCAGATTTTCACAAACCCGA | GACCGATTTGACTCAGACCA |
Thil2 | TCTCAGCCGTTGCCATCCTC | CCGCTCGCTCTCTACCTCAC |
Thil3 | TAAAGCCAAGAAGCCAACCA | CATTACAGAAACGGGCACAT |
Thil4 | CAAGCAACTCTCAAGAACAC | TTACAGTTACAGGCACACGA |
Thil5 | ATCTTAGAGCCATTCATTTC | TGACATATACTGGTCTTGGA |
Thil6 | ATAAATAGGAGCCTCGTGTT | CATTACAGAAACGGGCACAT |
Thil7 | CACCCAACCCTGACACACTC | CAGCAACTCTGAGGACGAAC |
Gene | Forward primer(5'-3') | Reverse primer(5'-3') |
---|---|---|
Thil1 | ATGGCGTCGTCGCAGAAGTTCTTC | ACATGGCTTGGTGCACATGCACT |
Thil2 | ATGGAGTCATCACACAAGCTTTTC | GCACTGCTTGGTGCACATGCAGCT |
Thil3 | ATGGCACCCAGCAAGAGTATTAAG | GGCTTCAACAGACTGAATGACTG |
Thil6 | ATGGCACCCAGCAAGAGTATTAAG | GGCTTCAACAGACTGAATGACTGC |
Thil7 | ATGGAGGCTTCACGCAAGCTCCTC | GTAGCGGCAGTGGGTGGTGCAGA |
Table 2 Primer sequences for Thil gene cloning
Gene | Forward primer(5'-3') | Reverse primer(5'-3') |
---|---|---|
Thil1 | ATGGCGTCGTCGCAGAAGTTCTTC | ACATGGCTTGGTGCACATGCACT |
Thil2 | ATGGAGTCATCACACAAGCTTTTC | GCACTGCTTGGTGCACATGCAGCT |
Thil3 | ATGGCACCCAGCAAGAGTATTAAG | GGCTTCAACAGACTGAATGACTG |
Thil6 | ATGGCACCCAGCAAGAGTATTAAG | GGCTTCAACAGACTGAATGACTGC |
Thil7 | ATGGAGGCTTCACGCAAGCTCCTC | GTAGCGGCAGTGGGTGGTGCAGA |
基因 Gene | 基因ID Gene ID | 染色体 Chromosome | 氨基酸数目 Number of amino acids | 分子量 Molecular weight/kD | 理论等电点Theoretical pI | 亲水性总平均值 Grand average of hydrophilicity | 脂肪族氨基酸指数 Aliphatic index |
---|---|---|---|---|---|---|---|
Thil1 | HORVU1G007680 | 2 | 231 | 18.83 | 5.3 | 0.748 | 19.05 |
Thil2 | HORVU1G007650 | 2 | 227 | 18.53 | 5.3 | 0.719 | 17.18 |
Thil3 | HORVU1G112060 | 5 | 279 | 23.23 | 5.31 | 0.658 | 24.73 |
Thil4 | HORVU1G097030 | 5 | 300 | 24.96 | 5.20 | 1.055 | 19.33 |
Thil5 | HORVU1G002760 | 1 | 414 | 34.23 | 5.22 | 0.798 | 25.60 |
Thil6 | HORVU1G001100 | 6 | 348 | 29.08 | 5.27 | 0.726 | 26.72 |
Thil7 | HORVU1G082390 | 4 | 249 | 20.62 | 5.23 | 0.911 | 12.45 |
Table 3 Basic information of Thil family genes from barley
基因 Gene | 基因ID Gene ID | 染色体 Chromosome | 氨基酸数目 Number of amino acids | 分子量 Molecular weight/kD | 理论等电点Theoretical pI | 亲水性总平均值 Grand average of hydrophilicity | 脂肪族氨基酸指数 Aliphatic index |
---|---|---|---|---|---|---|---|
Thil1 | HORVU1G007680 | 2 | 231 | 18.83 | 5.3 | 0.748 | 19.05 |
Thil2 | HORVU1G007650 | 2 | 227 | 18.53 | 5.3 | 0.719 | 17.18 |
Thil3 | HORVU1G112060 | 5 | 279 | 23.23 | 5.31 | 0.658 | 24.73 |
Thil4 | HORVU1G097030 | 5 | 300 | 24.96 | 5.20 | 1.055 | 19.33 |
Thil5 | HORVU1G002760 | 1 | 414 | 34.23 | 5.22 | 0.798 | 25.60 |
Thil6 | HORVU1G001100 | 6 | 348 | 29.08 | 5.27 | 0.726 | 26.72 |
Thil7 | HORVU1G082390 | 4 | 249 | 20.62 | 5.23 | 0.911 | 12.45 |
Fig. 7 Subcellular localization of Thil in barley The selected Thil-GFP fusion protein as well as GFP-empty as the control were independently transiently expressed in tobacco leaves and imaged under a confocal microscope. Bars=20 μm
[1] | 吕夏晨, 徐玲, 张蓝天, 等. 褪黑素对干旱胁迫下大麦生理及蜡质基因表达的影响[J]. 植物生理学报, 2020, 56(5):1073-1080. |
LV XC, Xu L, Zhang LT, et al. Effects of exogenous melatonin on physiology and waxy genes expression in barley under drought stress[J]. Plant Physiol J, 2020, 56(5):1073-1080. | |
[2] | 汪军成, 孟亚雄, 徐先良, 等. 大麦苗期抗旱性鉴定及评价[J]. 干旱地区农业研究, 2013, 31(4):135-143. |
Wang JC, Meng YX, Xu XL, et al. Identification and assessment on drought-resistance of Hordeum vulgare L. at seedling stage[J]. Agric Res Arid Areas, 2013, 31(4):135-143. | |
[3] | 鞠乐, 齐军仓, 贺雪, 等. 大麦种子萌发期抗旱性鉴定指标的筛选及抗旱性评价[J]. 新疆农业科学, 2016, 53(11):2008-2014. |
Ju L, Qi JC, He X, et al. Screening drought resistance identification index and drought resistance evaluation in barley during seed germination period[J]. Xinjiang Agric Sci, 2016, 53(11):2008-2014. | |
[4] |
周元成, 曹永立, 王镇, 等. 不同大麦品种抗旱性鉴定指标的筛选与评价[J]. 中国农业科技导报, 2022, 24(2):86-92.
doi: 10.13304/j.nykjdb.2020.0825 |
Zhou YC, Cao YL, Wang Z, et al. Screening and evaluation of drought resistance indexes in different barley varieties[J]. J Agric Sci Technol, 2022, 24(2):86-92. | |
[5] |
Holtorf S, Ludwig-Müller J, Apel K, et al. High-level expression of a viscotoxin in Arabidopsis thaliana gives enhanced resistance against Plasmodiophora brassicae[J]. Plant Mol Biol, 1998, 36(5):673-680.
pmid: 9526499 |
[6] |
Broekaert WF, Terras FR, Cammue BP, et al. Plant defensins:novel antimicrobial peptides as components of the host defense system[J]. Plant Physiol, 1995, 108(4):1353-1358.
pmid: 7659744 |
[7] |
García-Olmedo F, Molina A, Alamillo JM, et al. Plant defense peptides[J]. Biopolymers, 1998, 47(6):479-491.
doi: 10.1002/(SICI)1097-0282(1998)47:6<479::AID-BIP6>3.0.CO;2-K pmid: 10333739 |
[8] |
Bohlmann H, Apel K. Thionins[J]. Annu Rev Plant Physiol Plant Mol Biol, 1991, 42:227-240.
doi: 10.1146/annurev.pp.42.060191.001303 URL |
[9] |
Almaghrabi B, Ali MA, Zahoor A, et al. Arabidopsis thionin-like genes are involved in resistance against the beet-cyst nematode(Heterodera schachtii)[J]. Plant Physiol Biochem, 2019, 140:55-67.
doi: 10.1016/j.plaphy.2019.05.005 URL |
[10] |
Bohlmann H, Broekaert W. The role of thionins in plant protection[J]. Crit Rev Plant Sci, 1994, 13(1):1-16.
doi: 10.1080/07352689409701905 URL |
[11] |
Herker E, Jungwirth H, Lehmann KA, et al. Chronological aging leads to apoptosis in yeast[J]. J Cell Biol, 2004, 164(4):501-507.
pmid: 14970189 |
[12] |
Bulet P, Stöcklin R, Menin L. Anti-microbial peptides:from invertebrates to vertebrates[J]. Immunol Rev, 2004, 198:169-184.
doi: 10.1111/j.0105-2896.2004.0124.x URL |
[13] |
Oh BJ, Ko MK, Kostenyuk I, et al. Coexpression of a defensin gene and a thionin-like via different signal transduction pathways in pepper and Colletotrichum gloeosporioides interactions[J]. Plant Mol Biol, 1999, 41(3):313-319.
pmid: 10598099 |
[14] |
Epple P, Apel K, Bohlmann H. An Arabidopsis thaliana thionin gene is inducible via a signal transduction pathway different from that for pathogenesis-related proteins[J]. Plant Physiol, 1995, 109(3):813-820.
pmid: 8552715 |
[15] |
Epple P, Apel K, Bohlmann H. Overexpression of an endogenous thionin enhances resistance of Arabidopsis against Fusarium oxysporum[J]. Plant Cell, 1997, 9(4):509-520.
pmid: 9144959 |
[16] |
Epple P, Vignutelli A, Apel K, et al. Differential induction of the Arabidopsis thaliana Thi2. 1 gene by Fusarium oxysporum f. sp. matthiolae[J]. Mol Plant Microbe Interact, 1998, 11(6):523-529.
doi: 10.1094/MPMI.1998.11.6.523 URL |
[17] |
Plattner S, Gruber C, Stadlmann J, et al. Isolation and characterization of a thionin proprotein-processing enzyme from barley[J]. J Biol Chem, 2015, 290(29):18056-18067.
doi: 10.1074/jbc.M115.647859 pmid: 26013828 |
[18] | 李月颖, 李菁, 刘长宁. 蒺藜苜蓿SBP-box转录因子基因家族全基因组分析[J]. 湖南师范大学自然科学学报, 2017, 40(6):24-33, 95. |
Li YY, Li J, Liu CN. Genome-wide analysis of SBP-box transcription factor gene family in Medicago truncatula[J]. J Nat Sci Hunan Norm Univ, 2017, 40(6):24-33, 95. | |
[19] |
Silverstein KAT, Moskal WA Jr, Wu HC, et al. Small cysteine-rich peptides resembling antimicrobial peptides have been under-predicted in plants[J]. Plant J, 2007, 51(2):262-280.
pmid: 17565583 |
[20] |
Bohlmann H, Clausen S, Behnke S, et al. Leaf-specific thionins of barley-a novel class of cell wall proteins toxic to plant-pathogenic fungi and possibly involved in the defence mechanism of plants[J]. EMBO J, 1988, 7(6):1559-1565.
doi: 10.1002/j.1460-2075.1988.tb02980.x pmid: 16453847 |
[21] | 张知晓, 泽桑梓, 户连荣, 等. 茉莉酸甲酯生物活性研究进展[J]. 河南农业科学, 2018, 47(11):1-7. |
Zhang ZX, Ze SZ, Hu LR, et al. Research Progress on bioactivity of methyl jasmonate[J]. Henan Agricultural Science, 2018, 47(11):1-7. |
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