Isolation and Identification of Alicyclobacillus A1 and Its Bio-solubilization of Middle- and Low-grade Rock Phosphates

doi:10.13560/j.cnki.biotech.bull.1985.2016.11.026

Abstract

Abstract: Bio-solubilization of phosphorous has pioneered a novel approach to make full use of rich but refractory middle- and low-grade phosphate ores in China,and this work aims to screen high-efficiency phosphorous-solubilizing bacteria for utilization of middle- and low-grade phosphate ores. A facultative heterotrophic strain A1 was isolated from the acid mine drainage of Yichang phosphate mines of Hubei province,and was identified as Alicyclobacillus aeris according to its morphological,physiological and biochemical characteristics,and 16S rDNA sequence. For strain A1,YSG medium was optimal among four media regarding bacterial growth and its bio-solubilization of phosphorous from phosphate ores;the fraction of phosphorous leached by strain A1 reached 77.22%,which increased about 62% compared to chemical leaching of abiotic control. According to X-ray diffraction（XRD）analyses of raw phosphate ores and bioleached residues in YSG medium as well as high performance liquid chromatography of the leachate,soluble phosphates were released by dissolving fluorapatite and carbonate-fluorapatite in phosphate ores via organic acid mixture（mainly oxilic acid and citric acid）generated during the fermentation of strain A1 with organic carbon sources,and simultaneously gypsum was formed.

Key words: Alicyclobacillus aeris, isolation, identification, middle- and low-grade rock phosphates, bioleaching

LI Ling-ling, LÜ Zao-sheng, YANG Zhong-hua, ZUO Zhen-yu, LI Yao-yi. Isolation and Identification of Alicyclobacillus A1 and Its Bio-solubilization of Middle- and Low-grade Rock Phosphates[J]. Biotechnology Bulletin, 2016, 32(11): 224-234.

References

[1] 樊蕾, 方晓峰. 我国中低品位磷矿利用技术现状及前景展望[J]. 化学矿物及加工, 2015, 8：42-46.
[2] 张琛, 郑红艾, 周笑绿, 等. 生物浸出技术的发展及其电化学研究现状[J]. 金属矿山, 2014, 12：122-128.
[3] 孟春瑜, 刘文彦, 刘兴宇, 等. Alicyclobacillus属细菌及其在生物冶金中的研究进展[J]. 矿冶, 2015, 24（4）：43-48.
[4] 吴学玲, 朱若林, 丁建南, 等. 嗜酸热异养菌TC-2的分离鉴定及其对微生物浸矿的影响[J]. 中国有色金属学报, 2008, 18（12）：2259-2264.
[5] Yahya A, Hallberg KB, Johnson DB. Iron and carbon metabolism by a mineral-oxidizing Alicyclobacillus-like bacterium[J]. Archives of Microbiology, 2008, 189（4）：305-312.
[6] Wu X, Zhang Z, Liu L, et al. Metal resistance-related genes are differently expressed in response to copper and zinc ion in six Acidithiobacillus ferrooxidans strains[J]. Current Microbiology, 2014, 69（6）：775-784.
[7] 朱小翠, 樊明涛. 陕西省苹果汁中脂环酸芽孢杆菌的分离与鉴定[J]. 西北农林科技大学学报：自然科学版, 2008, 36（6）：189-194.
[8] 白淼, 张灿, 郑文杰, 等. 脂环酸芽孢杆菌的分离鉴定与控制技术研究进展[J]. 环境与健康杂志, 2015, 32（7）：650-654.
[9] 胡贻椿, 岳田利, 袁亚宏, 等. 浓缩苹果汁车间空气中嗜酸耐热菌的分离鉴定研究[J]. 农产品加工（学刊）, 2007, 3：11-13.
[10] Joe SJ, Suto K, Inoie C, et al. Isolation and characterization of acidophilic heterotrophic iron-oxidizing bacterium from enrichment culture obtained from acid mine drainage treatment plant[J]. J Biosci Bioeng, 2007, 104（2）：117-123.
[11] 袁亚宏, 刘晓珂, 岳田利, 等. 一株源于果园Alicyclobacillus的分离、鉴定及其生物学特性[J]. 食品科学, 2012, 33（17）：129-135.
[12] Guo X, You XY, Liu LJ, et al. Alicyclobacillus aeris sp. nov. a novel ferrous- and sulfur-oxidizing bacterium isolated from a copper mine[J]. International Journal of Systematic and Evolutionary Microbiology, 2009, 59（Pt10）：2415-2420.
[13] 李凌凌, 吕早生, 吴敏, 等. 重组的葡萄糖脱氢酶催化辅酶的再生性质[J]. 华中科技大学学报：自然科学版, 2010, 38（3）：112-115.
[14] Li L, Lv Z, Yuan X, et al. Effect of L-glycine on bioleaching of collophanite by Acidithiobacillus ferrooxidans[J]. International Biodeterioration & Biodegradation, 2013, 85（7）：156-165.
[15] 王树林, 黄志良, 罗欣, 等. 宜昌磷矿工艺矿相学研究[J]. 武汉工程大学学报, 2012, 34（2）：55-59.
[16] Tuovinen OH, Bhatti TM, Bigham JM, et al. Oxidative dissolution of arsenopyrite by mesophilic and moderately thermophilic acidophiles[J]. Appl Environ Microbiol, 1994, 60（9）：3268-3274.
[17] 银婷婷, 王敬敬, 柳影, 等. 高效解磷菌的筛选及其促生机制的初步研究[J]. 生物技术通报, 2015, 31（12）：234-242.
[18] Bhatti TM, Yawar W. Bacterial solubilization of phosphorus from phosphate rock containing sulfur-mud[J]. Hydrometallurgy, 2010, 103（1-4）：54-59.
[19] Dorozhkin SV. Dissolution mechanism of calcium apatites in acids：A review of literature[J]. World Journal of Methodology, 2012, 2（1）：1-17.
[20] Delvasto P, Valverde A, Ballester A, et al. Characterization of brushite as a recrystallization product formed during bacterial solubilization of hydroxyapatite in batch cultures[J]. Soil Biology & Biochemistry, 2006, 38：2645-2654.
[21] 刘波, 王阶平, 陶天申, 等. 芽孢杆菌属及其近缘属种名目录[J]. 福建农业学报, 2015, 30（1）：38-59.
[22] 赵小蓉, 林启美, 李保国. 微生物溶解磷矿粉能力与pH及分泌有机酸的关系[J]. 微生物学杂志, 2003, 23（3）：5-7.
[23] Chen Z, Ma S, Liu LL. Studies on phosphorous solubilizing activity of a strain of phosphobacteria isolated from chestnut type oil in China[J]. Bioresource Technol, 2008, 99（14）：6702-6707.