Biosynthesis of 3,4-dihydroxymandelic Acid in an Engineered Escherichia coli Strain

doi:10.13560/j.cnki.biotech.bull.1985.2017.01.015

Abstract

Abstract: 3,4-dihydroxymandelic acid,an important pharmaceutical and spices intermediate,possesses strong antioxidative and radical scavenging activities. To achieve the de novo biosynthesis of 3,4-dihydroxymandelic acid in Escherichia coli,the genes of hydroxymandelate synthase（HmaS）from Streptomyces coelicolor and 4-hydroxyphenylacetate 3-hydroxylase（HpaBC）from E. coli were cloned into pTrcHisB and overexpressed in an engineered E. coli strain MG1655/ΔA. After induction of IPTG and optimization of the inducing temperature,the yield of 3,4-dihydroxymandelic acid reached 240 mg/L in shake flask after 36 hours fermentation,laying a foundation for the large-scale fermentation production of 3,4-dihydroxymandelic acid .

Key words: Escherichia coli, 3,4-dihydroxymandelic acid, biosynthesis, HmaS, HpaBC

LI Xiao-lin, ZHOU Wei, ZHUANG Yi-bin, LU Fu-ping, YIN Hua. Biosynthesis of 3,4-dihydroxymandelic Acid in an Engineered Escherichia coli Strain[J]. Biotechnology Bulletin, 2017, 33(1): 135-140.

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