CRISPR/Cas Genome Editing Technology and Its Application in Fungi

doi:10.13560/j.cnki.biotech.bull.1985.2017.03.009

Abstract

Abstract: CRISPR/Cas system is a self-defense system against exogenous virus or plasmid in bacteria and archaea. CRISPR/Cas9,an expeditious genome editing technology,was developed according to the type II CRISPR/Cas system. Nowadays,the CRISPR/Cas9 genome editing technology has broadly utilized in gene function and genetic modification research in animal,plant and microorganism. Here,we briefly introduce the structure,mechanism and classification of CRISPR/Cas system,describe the application of CRISPR/Cas9 genome editing technology in fungi,and analyze the advantages,disadvantages as well as the prospect and application values of this technology. It is aimed at providing a useful reference for researchers in genetic modification of fungi.

Key words: fungi, genome editing technology, CRISPR/Cas9, gene function

YIN Chao-min, FAN Xiu-zhi, SHI De-fang, GAO Hong. CRISPR/Cas Genome Editing Technology and Its Application in Fungi[J]. Biotechnology Bulletin, 2017, 33(3): 58-65.

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