Suspensor PCD Induced by GA in Nicotiana tabacum Under In Vitro Culture

doi:10.13560/j.cnki.biotech.bull.1985.2017.03.011

Abstract

Abstract: Programmed cell death（PCD）is ubiquitous in the entire developmental process of plant,and hormones are a key regulating factor to PCD in plant. To investigate the role of GA in the suspensor PCD,systems of in vitro culture of the ovule and embryo were well established. By setting different concentrations of GA and culture time,100 μmol/L GA was appropriate and embryos may not be cultured for more than 3 days. The suspensor PCD was efficiently induced by exogenous GA during in vitro culture of embryo. As the concentration of GA increased,the proportion of PCD in suspensor cells gradually raised. Howerver,excessively high concentration of GA and long culture time would also induce abnormal PCD in porper cells.

Key words: suspensor, PCD, GA, in vitro culture

LUO An, CHEN Ke-qiang. Suspensor PCD Induced by GA in Nicotiana tabacum Under In Vitro Culture[J]. Biotechnology Bulletin, 2017, 33(3): 71-77.

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