Biotechnology Bulletin ›› 2017, Vol. 33 ›› Issue (3): 78-85.doi: 10.13560/j.cnki.biotech.bull.1985.2017.03.012

Previous Articles     Next Articles

Proteomic Analysis on Tuberous Roots of Cassava Cultivar ZM-Seaside and Mosaic-leaf Mutation

SONG Yan-chao1,2, An Fei-fei2, Xue Jing-jing2, Qin Yu-ling2, Li Kai-mian2, CHEN Song-bi2   

  1. 1. Hainan University,Haikou 570228;
    2. Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Conservation and Utilization of Cassava Genetic Resources Ministry of Agriculture,Danzhou 571737
  • Received:2016-07-01 Online:2017-03-26 Published:2017-03-07

PDF (PC)

464

Abstract: In order to study yield differences between cassava cultivar ZM-Seaside（high yield）and mosaic-leaf mutation（low yield）,the tuberous roots were analyzed from the aspects of agronomic traits and proteomics in the present study. The results will provide a theoretical basis for the selection of high yield cassava varieties. Starch content was determined by polarimetry,and the hydrocyanic acid concentration was measured by silver nitrate titration method. Protein extract were performed by phenol extraction and protein was separated using two-dimensional electrophoresis. Delta 2D software was used to determine the differentially expressed proteins. The differentially expressed proteins were identified using mass spectrometry in combination with the KEGG database to classify proteins according to their functions. Western blot was used to analyze the protein expression level. String online software was used to construct protein-protein interaction network. Results showed that the starch content of ZM-Seaside（29.18%）was significantly higher than that of mosaic-leaf mutation（25.83%）. Hydrocyanic acid contents of two cassava genotypes in flesh were less than 50 mg/kg,and both of them are in the range of edible cassava. The dry matter rate of ZM-Seaside was 40.28%,significant higher that of mosaic-leaf mutation（37.16%）. 39 differentially expressed protein spots were detected in the tuberous root of ZM-Seaside compared with mosaic-leaf mutation,of which 23 were up-regulated,16 were down-regulated. 28 protein spots were successfully identified,in which their functions related to carbohydrate and energy metabolism（7）,chaperones（8）,detoxifying and antioxidant（2）,protein biosynthesis（1）,structure（3）and function unknown proteins（7）. STRING metabolic network showed that Heat shock protein and Molecular chaperone Hsp90-1 were the hub proteins,which probably are the key of the whole regulatory network. They would be the key proteins affecting the yields between mosaic-leaf mutation and ZM-Seaside,suggesting they may use as the marked proteins to select high-yield cassava varieties.

Key words: Cassava cultivar, mosaic-leaf mutation, tuberous root, agronomic traits, proteomics