Proteomics Analysis of Ammopiptanthus mongolicus Leaves Under Drought Stress

doi:10.13560/j.cnki.biotech.bull.1985.2017-0012

Abstract

Abstract: The aim of the present study is to reveal the proteomic variation of Ammopiptanthus mongolicus（Maxim）Cheng. f leaves under drought stress. The plants were exposed to 20% PEG 6000 for 1 and 72 h,and 0 h as control. The extracted leaves proteins of the treatment and the control were analyzed by two-dimensional electrophoresis（2-DE）,and the differentially expressed proteins were identified by MALDI-TOF-TOF. In total,40 differentially expressed proteins were detected. These identified proteins were mainly involved in photosynthesis,ROS scavenging,protein synthesis,processing and degradation,transport,defense related,RNA processing,amino acid metabolism,other and unknown function. Conclusively,the key of A. mongolicus response to drought stress was the maintenance of structure and photosynthesis of chloroplasts.

Key words: Ammopiptanthus mongolicus, leaf, proteome, differentially expressed proteins

FU Chen-xi, XIAO Zi-hua, GAO Fei, ZHOU Yi-jun. Proteomics Analysis of Ammopiptanthus mongolicus Leaves Under Drought Stress[J]. Biotechnology Bulletin, 2017, 33(6): 69-80.

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