Isolation and Identification of a Zinc-resistant Strain and Effect of Its Characteristics on the Remediation Efficiency of Brassica juncea in Zinc-polluted Soil

doi:10.13560/j.cnki.biotech.bull.1985.2017-0587

Abstract

Abstract:

A zinc-resistant strain was isolated from heavy metal-polluted soil，and identified as Bacillus. Its performance of removing Zn²⁺ under different experimental conditions was studied. The results showed that：the removal efficiency of Zn²⁺ was the best，and the removal rate of Zn²⁺ was 94% in 12 h when the initial concentration of Zn²⁺ was 300 mg/L，the temperature was 37℃，inoculation amount was 5%. Subsequently，a pot experiment was conducted for investigating the influence of adding the different concentration of the strain on plant growth and Zn uptake by Brassica juncea. The results showed that the Zn content in ground and underground part of B. juncea increased by 56.1% and 24.3% respectively while adding 40 mL bacterial liquid（1×10⁹ CFU/mL），compared with the control group. This is mainly due to the strain improves the zinc content under weak acid extraction condition.

Key words: zinc-resistant strain, screening, Zn²⁺removal efficiency, Brassica juncea

CHEN Yang, SONG Tian-shun, ZHOU Guo-ling, XIE Jing-jing. Isolation and Identification of a Zinc-resistant Strain and Effect of Its Characteristics on the Remediation Efficiency of Brassica juncea in Zinc-polluted Soil[J]. Biotechnology Bulletin, 2017, 33(10): 156-162.

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