Identification of NBS-LRR-like Disease-resistant Genes in Cinnamomum camphora and Clone of Two CcRNL Genes

doi:10.13560/j.cnki.biotech.bull.1985.2017-0570

Abstract

Abstract: Cinnamomum camphora is an germplasm resource with excellent resistance against pathogens in nature. Based on transcriptome data of C. camphora leaf tissue,the disease-resistant genes（R）of NBS（nucleotide-binding site）family were identified,and the physic-chemical properties,motifs and phylogenetic relationship of encoded putative proteins were analyzed through bioinformatics analysis. As results showed,28 NBS-like disease-resistant genes with complete encoding frame were identified from leaf transcriptome of C. camphora leaf tissue. The lengths of ORF ranged in 2 118-3 378 bp,and the estimated isoelectric point of putative proteins were in 5.35-9.03. The 8 motifs were identified in the NBS domain. The 28 R genes were divided into 5 subgroups based on the phylogenetic analysis. Additionally,2 RNL genes were cloned by PCR and designated as CcRNL1 and CcRNL2. The two genes shared 71% similarity in amino acid sequence,and there was RPW8-like conservative motif in the N-terminal of the polypeptides,indicating that they might have broad-spectrum resistance to powdery mildew as gene RPW8 in Arabidopsis thaliana has. Analyzing the young leaves from powdery mildew-infected C. camphora and normal one,the results revealed that CcRNL1 expressed by the induction of powdery mildew in C. camphora,therefore it was inferred to be involved in the response to stress process of powdery mildew pathogens.

Key words: Cinnamomum camphora, NBS-like disease-resistant gene, powdery mildew, expression analysis

ZHENG Yong-jie, WU Yan-fang, LI Jiang, ZHANG Ting, WANG Xin-dong. Identification of NBS-LRR-like Disease-resistant Genes in Cinnamomum camphora and Clone of Two CcRNL Genes[J]. Biotechnology Bulletin, 2018, 34(2): 142-149.

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