The bacteriostatic activity and control effects of 56 extracts from Chinese traditional medicine were screened and determined in order to acquire the ingredients that may effectively suppress the mycelial growth of Verticillium dahlia and increase the control effects on cotton wilt disease. The results of growth rate showed that the inhibition rate on V. dahlia mycelial growth by 26 of all tested 56 extracts was up to 50%,which were mainly distributed in 9 medicinal plants of Schisandra chinensis(Turcz.)baill,Schisandra propinqua var. sinensis,Manglietiastrum sinicum,Euphorbia sikkimensics,etc. Further,the cotton plants were treated by the extracts and the statistics of disease index and control effect was conducted. The results explained that the chloroform:acetone(3∶2,V/V)extract of S. chinensis(sampled from Heilongjiang province)presented significant control effects to cotton wilt disease. The stem dissection revealed that the extract effectively suppressed the browning of vascular bundle caused by wilt disease,and the symptom of leaf-falling from wilt disease in whole plant was significantly alleviated. The above data indicate that the chloroform:acetone(3∶2,V/V)extract from S. chinensis not only effectively inhibits the growth of V. dahlia in vitro,but also induces the resistance in cotton,thus decreases the browning of vascular bundle and alleviates the occurrence of cotton wilt disease..