Analysis of the Genetic Composition in Saccharina japonica and Undaria pinnatifida Based on SSR Markers

doi:10.13560/j.cnki.biotech.bull.1985.2017-0785

Abstract

Abstract: Saccharina japonica and Undaria pinnatifida varieties with higher purity and better character that could be cultivated by the first generation screening of gametophyte and spore seedlings. Using 11 seedling combinations of S. japonica and u. pinnatifida and based on a number of primers that may distinguish male and female parents of each group，the genetic composition of their parents and progeny were analyzed. Thirteen pairs of SSR markers which were able to distinguish male and female parents of 11 combinations were selected in the experiment. Results showed that among the 2013 seedlings，all progenies were the offspring of their parents in No. 48 combination，but only one of progenies was the offspring of their parents in No. 49 combination. Parents were genetically identical to all progenies in No. 22 combination of 2014 seedlings. Among all hybridized combinations of S. japonica and u. pinnatifida in 2015 seedlings No.36，No.38，No.39，No.41 were hybridized successfully，while other combinations were not so. There were no biological relationships between seven of progenies and their parents in No. 59 combination of 2016 seedlings. Analyzing the genetic composition of all individuals and parents in these 11 combinations revealed that some individuals were progenies of their parents，but the genetic composition of some individuals were inconsistent with their parents.

Key words: Saccharina japonica, Undaria pinnatifida, SSR markers, parents, genetic composition

PENG Jie, CUI Cui-ju, LIANG Guang-jin, LI Yan, WU Rui-na, LIU Yan-ling, TIAN Ping-ping, LI Xiao-jie. Analysis of the Genetic Composition in Saccharina japonica and Undaria pinnatifida Based on SSR Markers[J]. Biotechnology Bulletin, 2018, 34(3): 156-162.

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